He Shan, Wu Yue, Lu Kaixian, Zhu Heng, Wang Xuan, Qin Yaoyao, Li Huan, Zeng Lin, Han Jiaojiao, Zhou Xiangyang, Zhang Bin, Tang Bo
College of Food and Bioengineering, Bengbu University, Bengbu, China.
School of Marine Sciences, Ningbo University, Ningbo, China.
Front Pharmacol. 2025 Mar 26;16:1498358. doi: 10.3389/fphar.2025.1498358. eCollection 2025.
Sheep placenta extract (SPE) is a representative traditional medicinal substance that exhibits multiple experimentally validated physiological properties, including anti-aging effects, wound healing acceleration, antioxidant activity, and anti-inflammatory mechanisms. However, the mechanism by which SPE influences the delay of aging is still not yet clear.
Exploring the effects of sheep placenta extract on D-gal induced senescence in a mouse model of aging by macrogenomics and metabolomics.
In the serum of aging mice treated with SPE, the levels of antioxidant function such as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were notably higher compared to those in the blank group, whereas malondialdehyde (MDA) levels decreased. We revealed that SPE alleviated the changes in gut microbiota caused by aging in mice, with a significant decrease in the (F/B) ratio in the gut. Furthermore, (), which is known for its regulating immune response and potential anti-aging effects, showed a significant increase of 1177.94%. The analysis of UHPLC-QE-MS combined with orthogonal partial least squares discriminant analysis (OPLS-DA) screening of differential metabolites in mouse serum metabolic profiles revealed a significant upregulation of -5,8,11,14,17-eicosapentaenoic acid (EPA) and triptolide in serum metabolites, following SPE treatment, which are commonly believed to have immunosuppressive, anti-inflammatory, anti-proliferative, and anti-tumor effects.
The role of SPE in ameliorating aging may be associated with the increased abundance of A. muciniphila in the gut microbiota and the accumulation of two metabolites, EPA and triptolide, in the serum.
羊胎盘提取物（SPE）是一种典型的传统药物，具有多种经实验验证的生理特性，包括抗衰老作用、加速伤口愈合、抗氧化活性和抗炎机制。然而，SPE影响衰老延迟的机制仍不清楚。
通过宏基因组学和代谢组学探索羊胎盘提取物对D-半乳糖诱导的衰老小鼠模型衰老的影响。
与空白组相比，用SPE处理的衰老小鼠血清中，超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）等抗氧化功能水平显著更高，而丙二醛（MDA）水平降低。我们发现，SPE减轻了衰老引起的小鼠肠道微生物群变化，肠道中（F/B）比值显著降低。此外，以调节免疫反应和潜在抗衰老作用而闻名的（）显示显著增加了1177.94%。超高效液相色谱-四极杆飞行时间质谱联用（UHPLC-QE-MS）结合正交偏最小二乘法判别分析（OPLS-DA）对小鼠血清代谢谱中的差异代谢物进行筛选分析发现，SPE处理后，血清代谢物中-5,8,11,14,17-二十碳五烯酸（EPA）和雷公藤内酯醇显著上调，通常认为它们具有免疫抑制、抗炎、抗增殖和抗肿瘤作用。
SPE在改善衰老方面的作用可能与肠道微生物群中嗜黏蛋白阿克曼菌丰度增加以及血清中两种代谢物EPA和雷公藤内酯醇的积累有关。
