Chien D S, Schoenwald R D
J Pharm Sci. 1985 May;74(5):562-4. doi: 10.1002/jps.2600740515.
A sensitive method is described for the rapid determination of phenylephrine hydrochloride in human plasma. Phenylephrine was analyzed using high-performance liquid chromatography (HPLC) with fluorecence detection at excitation and emission wavelengths of 270 and 305 nm, respectively. A retention time of approximately 4.25 min was observed on chromatograms. Drug was separated from interfering plasma components by the use of disposable solid-phase extraction columns. Linear standard curves were constructed from drug concentrations of 0.5-5.0 and 2.5-80 ng/mL, for which an average recovery of 76-78% was obtained. Phenylephrine could be quantitatively measured at levels as low as 0.5 ng/mL. An assay precision (CV) of 23.6 and 15.9% was obtained at the lower sensitivities of 0.5 and 1.0 ng/mL, respectively. An example of the method was given for analysis of human plasma levels of drug following topical application of two drops of 2.5% aqueous or 10% viscous opthalmic solution to the eyes of two subjects.
描述了一种用于快速测定人血浆中盐酸去氧肾上腺素的灵敏方法。使用高效液相色谱（HPLC）对去氧肾上腺素进行分析，荧光检测的激发波长和发射波长分别为270nm和305nm。在色谱图上观察到保留时间约为4.25分钟。通过使用一次性固相萃取柱将药物与干扰性血浆成分分离。由0.5 - 5.0和2.5 - 80 ng/mL的药物浓度构建线性标准曲线，其平均回收率为76 - 78%。去氧肾上腺素可在低至0.5 ng/mL的水平进行定量测定。在较低灵敏度0.5和1.0 ng/mL时，测定精密度（CV）分别为23.6%和15.9%。给出了该方法的一个示例，用于分析两名受试者眼部局部滴入两滴2.5%水性或10%粘性眼科溶液后血浆中的药物水平。
