Park Gi-Rim, Bae Seon-Hwa, Kang Beom-Kyu, Seo Jeong-Hyun, Oh Jae-Hyeon
Upland Crop Breeding Research Division, National Institute of Crop Science, Rural Development Administration, Miryang-si, Gyeongnam, Republic of Korea.
Fruit Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Iseo-myeon, Wanju-gun, Republic of Korea.
Front Genet. 2025 Mar 26;16:1564160. doi: 10.3389/fgene.2025.1564160. eCollection 2025.
Drought stress significantly reduces soybean yield, underscoring the need to develop drought-resistant varieties and identify the underlying genetic mechanisms. However, the specific genes and pathways contributing to drought tolerance remain poorly understood. This study aimed to identify candidate genes associated with drought tolerance in soybean using a recombinant inbred line (RIL) population derived from PI416937 and Cheongsang.
A quantitative trait loci (QTL) mapping study using a 180K high-quality SNP array and composite interval mapping on 140 recombinant inbred lines, coupled with RNA sequencing of treated and control groups, was conducted to identify candidate genes for drought tolerance in soybean.
Through QTL mapping and differential gene expression profiling, five candidate genes were identified, with two ( and ) highlighted as putative candidates based on functional annotations. These genes appear to play critical roles in stress tolerance, including ion homeostasis and the regulation of plasma membrane ATPase, as well as the synthesis of heat shock proteins (HSPs) that mitigate dehydration and thermal stress. These findings advance our understanding of the genetic basis of drought tolerance in soybean and provide valuable targets for breeding programs aimed at developing resilient cultivars.
干旱胁迫显著降低大豆产量，这凸显了培育抗旱品种和确定潜在遗传机制的必要性。然而，对于有助于耐旱性的具体基因和途径仍知之甚少。本研究旨在利用源自PI416937和清桑的重组自交系（RIL）群体，鉴定大豆中与耐旱性相关的候选基因。
使用180K高质量SNP阵列对140个重组自交系进行数量性状位点（QTL）定位研究，并结合复合区间作图，同时对处理组和对照组进行RNA测序，以鉴定大豆耐旱性的候选基因。
通过QTL定位和差异基因表达谱分析，鉴定出五个候选基因，其中两个（和）根据功能注释被突出为假定候选基因。这些基因似乎在胁迫耐受性中发挥关键作用，包括离子稳态和质膜ATP酶的调节，以及减轻脱水和热胁迫的热休克蛋白（HSP）的合成。这些发现推进了我们对大豆耐旱性遗传基础的理解，并为旨在培育抗逆品种的育种计划提供了有价值的目标。
