Westergaard L
J Reprod Fertil. 1985 May;74(1):113-8. doi: 10.1530/jrf.0.0740113.
Antral follicles from normally menstruating women and women in the third trimester of pregnancy were classified as healthy or atretic by flow cytometric DNA measurements on aspirated granulosa cells and by the concentration of steroids in the follicular fluid. The oocytes contained in these follicles were characterized as healthy or degenerating by their morphology at the light microscopic level. In 98% of the cases (61/62) morphologically healthy or degenerating oocytes were found in follicles which were classified as healthy or atretic respectively. In the normally menstruating women degenerative changes in the oocyte and the remainder of the follicle appeared to occur in synchrony. In pregnant women asynchrony was noted between the oocyte and the remainder of the follicle as follicular atresia progressed. The present study demonstrated the usefulness of flow cytometric DNA measurement in characterizing antral follicles of all sizes as healthy or more or less atretic.
通过对抽吸的颗粒细胞进行流式细胞术DNA测量以及卵泡液中类固醇的浓度，将正常月经周期女性和妊娠晚期女性的窦卵泡分为健康或闭锁卵泡。这些卵泡中所含的卵母细胞在光学显微镜水平上根据其形态被表征为健康或退化。在98%的病例（61/62）中，分别在被分类为健康或闭锁的卵泡中发现了形态学上健康或退化的卵母细胞。在正常月经周期的女性中，卵母细胞和卵泡其余部分的退化变化似乎同步发生。在孕妇中，随着卵泡闭锁的进展，卵母细胞与卵泡其余部分之间出现了不同步。本研究证明了流式细胞术DNA测量在将各种大小的窦卵泡表征为健康或或多或少闭锁方面的有用性。
