Pontiggia Luca, Yosef Hesham, Biedermann Thomas, Moehrlen Ueli
Tissue Biology Research Unit, Department of Pediatric Surgery, University Children's Hospital Zurich, Zurich, Switzerland.
Children's Research Center, University Children's Hospital Zurich, Zurich, Switzerland.
Methods Mol Biol. 2025;2922:209-227. doi: 10.1007/978-1-0716-4510-9_16.
Studying human skin biology can aid in understanding the pathophysiology of skin diseases and developing novel therapies, including tissue engineering approaches. Among various optical and spectroscopic techniques, Raman micro-spectroscopy stands out. This non-invasive method requires minimal sample preparation and provides molecular-level information on healthy and diseased cell types. The following chapter aims to provide a comprehensive guide to the basic methods of Raman micro-spectroscopy analysis of human epidermal samples from the perspective of their biochemical-molecular relationship, without the need for a deep and specialized knowledge in the field of spectroscopy. We do this based on two distinct examples: (1) Unstained cell subpopulations identified by hierarchical cluster analysis are compared with antibody treated sorted cell populations, and (2) skin cell type-specific molecular features are determined, and the composition of unknown cellular mixtures is revealed.
研究人类皮肤生物学有助于理解皮肤疾病的病理生理学并开发新的治疗方法，包括组织工程方法。在各种光学和光谱技术中，拉曼显微光谱脱颖而出。这种非侵入性方法所需的样品制备最少，并能提供关于健康和患病细胞类型的分子水平信息。以下章节旨在从生化 - 分子关系的角度，为人类表皮样品的拉曼显微光谱分析基本方法提供全面指南，而无需光谱领域的深入专业知识。我们通过两个不同的例子来做到这一点：（1）将通过层次聚类分析识别的未染色细胞亚群与抗体处理的分选细胞群体进行比较，以及（2）确定皮肤细胞类型特异性分子特征，并揭示未知细胞混合物的组成。
