Liu Haisheng, Wang Hongyu, Zhang Chao, Wang Meng, Liu Qian, Zhao Yaru, Yu Jiaxing, Zhao Guihong, Zhang Ming
College of Agriculture and Bioengineering, Heze University, Heze, Shandong, 274000, China.
Mikrochim Acta. 2025 Apr 10;192(5):292. doi: 10.1007/s00604-025-07080-6.
A G-quadruplex-based fluorescent biosensor is introduced that employs a self-complementary DNA probe for detection of hypochlorite ions (ClO) in the rostral ventrolateral medulla (RVLM) of rats afflicted with stress-induced hypertension (SIH). The biosensor capitalizes on the interaction between Thioflavin T (ThT) and guanine-rich DNA sequences, exhibiting exceptional sensitivity, specificity, and swift detection capabilities for ClO. Through optimization of the detection parameters, we achieved a minimal detection threshold of 0.0486 μM and a rapid reaction time of 5 min. The biosensor's high selectivity for ClO was confirmed by its lack of response to other reactive oxygen species (ROS). When applied to SIH rat models, the biosensor revealed markedly elevated ClO levels in the RVLM of SIH rats versus control rats, implicating ClO in the disease's pathophysiology. This label-free, G-quadruplex-based biosensor represents a significant advancement in the detection of ClO⁻, offering a highly sensitive and specific tool for monitoring reactive oxygen species in biological systems. This study not only deepens our comprehension of SIH but also offers a potent diagnostic and monitoring tool for ROS-related diseases, with implications for therapeutic intervention and disease management.
一种基于G-四链体的荧光生物传感器被引入，该传感器采用自互补DNA探针来检测应激性高血压（SIH）大鼠延髓头端腹外侧区（RVLM）中的次氯酸根离子（ClO）。该生物传感器利用硫黄素T（ThT）与富含鸟嘌呤的DNA序列之间的相互作用，对ClO表现出卓越的灵敏度、特异性和快速检测能力。通过优化检测参数，我们实现了0.0486 μM的最低检测阈值和5分钟的快速反应时间。该生物传感器对ClO的高选择性通过其对其他活性氧（ROS）无反应得到证实。当应用于SIH大鼠模型时，该生物传感器显示，与对照大鼠相比，SIH大鼠RVLM中的ClO水平显著升高，这表明ClO参与了该疾病的病理生理过程。这种基于G-四链体的无标记生物传感器代表了ClO⁻检测方面的重大进展，为监测生物系统中的活性氧提供了一种高度灵敏和特异的工具。这项研究不仅加深了我们对SIH的理解，还为与ROS相关的疾病提供了一种有效的诊断和监测工具，对治疗干预和疾病管理具有重要意义。
