Kong Wei-Chuang, Li Chen-Chen, Niu Jun-Tao, Liu Xu-Ying, Wei Dong, Kang Yan-Fei
College of Laboratory Medicine, Institute of Pathogen Biology and Immunology, Hebei Key Laboratory of Neuropharmacology, Hebei Key Laboratory of Quality & Safety Analysis-Testing for Agro-Products and Food and Zhang Jiakou Key Laboratory of Organic Light Functional Materials, Hebei North University, Zhangjiakou, Hebei, 075000, China.
Anal Methods. 2025 May 1;17(17):3471-3479. doi: 10.1039/d4ay02265c.
Enrofloxacin (ENR), a third-generation fluoroquinolone antibiotic, poses significant health risks due to potential drug residues in the food chain, necessitating effective monitoring methods. Here, we synthesized a G-quadruplex-targeted fluorescent probe PBTZM containing styrylbenzothiazole. Using this probe, we successfully developed an aptasensor for the detection of ENR by combining it with an ENR aptamer. Under optimal conditions, the aptasensor provided a linear range of 0.001-5 μM and a detection limit of 24.74 nM. In addition, this method showed good performance for ENR detection in river water, milk, serum and urine samples and was further validated by standard HPLC techniques without a significant difference ( > 0.05). It will have great potential in advancing research of quinolone antibiotics and the development of aptamer-based biosensing technologies.
