Li Haozhen, Wang Shuyao, Zhang Xiaohua, Song Kangkang, Yang Long
College of Plant Protection, Agricultural Big-Data Research Center and Key Laboratory of Agricultural Film Application of Ministry of Agriculture and Rural Affairs, Shandong Agricultural University, Tai'an 271018, China.
Bioresource Engineering, McGill University, Sainte-Anne-de-Bellevue, QC H9X 3V9, Canada.
Food Chem X. 2025 Mar 24;27:102410. doi: 10.1016/j.fochx.2025.102410. eCollection 2025 Apr.
Tea quality in greenhouse was certain gap with open air. Metabolites and foliar microorganisms were investigated under seaweed fertiliser (CF) and gibberellin (CH) treatments using sensory evaluation, HPLC, untargeted metabolomics, 16S rDNA, and Internal Transcribed Spacer. CF tea was mellow, less astringent, and of better quality compared to CH. Catechin, -(-)Epicatechin, and Epigallocatechin were notably lower in CF. Differentially accumulated metabolites (DAMs) were notably enriched in Flavonoid and Phenylpropanoid biosynthesis, both involved in Catechin synthesis. DAMs in these pathways appeared down-regulated in CF. The CF improved quality by down-regulating metabolites in Phenylpropanoid biosynthesis in conjunction with microbial community metabolism enriched in amino acid and secondary metabolite biosynthesis. Metabolite- microbial correlation analysis indicated that the highest correlation with phenylpropane pathway metabolites was in bacteria and , and in fungi . The study provides theoretical basis for regulating flavour quality of greenhouse tea.
温室环境下茶叶品质与露天环境存在一定差距。采用感官评价、高效液相色谱法、非靶向代谢组学、16S核糖体DNA和内转录间隔区等方法，对海藻肥（CF）和赤霉素（CH）处理下的代谢产物和叶片微生物进行了研究。与CH相比，CF茶口感醇厚，涩味较轻，品质更佳。CF茶中的儿茶素、-(-)表儿茶素和表没食子儿茶素含量显著较低。差异积累代谢物（DAMs）在类黄酮和苯丙烷生物合成中显著富集，这两个过程均参与儿茶素的合成。CF中这些途径中的DAMs似乎下调。CF通过下调苯丙烷生物合成中的代谢物，结合富含氨基酸和次生代谢物生物合成的微生物群落代谢来提高品质。代谢物与微生物的相关性分析表明，与苯丙烷途径代谢物相关性最高的是细菌和，以及真菌。该研究为调控温室茶风味品质提供了理论依据。
