Tyshko N V, Timoshenko K A, Shestakova S I, Zotov V A
Federal Research Centre of Nutrition, Biotechnology and Food Safety, 109240, Moscow, Russian Federation.
Vopr Pitan. 2025;94(1):100-109. doi: 10.33029/0042-8833-2025-94-1-100-109. Epub 2025 Feb 3.
The trend to the modern food industry's reorientation into the manufacturing of food with increased nutritional density, enriched, among other things, with complete protein, leads to the need to expand the raw material base and search for non-traditional protein sources, among which insects are considered to be a very promising resource. The insects species composition, the food use of which is approved in countries that previously had no tradition of using such raw materials, is represented by ubiquitous synanthropic organisms that have accompanied mankind for thousands of years - the mealybug (Tenebrio molitor), house cricket (A. domesticus) and others. In particular, A. domesticus belongs to polyphagous species that feed both plant and animal substrates, producing their own complete protein. Based on the results of our own research and analysis of literature data, the chemical composition of arthropods potentially used for food purposes requires careful control due to the possible risk of excessive content of mineral components. Both regulation of feed composition and feeding schedule of insects, taking into consideration their age and species affiliation, can be considered as risk management methods. of the research was the investigation of the influence of feeding schedule on the micronutrient (mineral) composition of house cricket A. domesticus biomass. . The A. domesticus house cricket produced by ONTO LLC was used for this research. Elemental analysis of 8 insect samples was carried out. The content of potassium, sodium, calcium, magnesium, manganese, cobalt, chromium, nickel, iron, copper, zinc, lead and cadmium in insect biomass samples was determined by atomic absorption spectroscopy. The element composition of cricket biomass (age of the 6th moult and adults) obtained under different feeding schedule was assessed from the view point of the possibility for using insects as a source of relevant mineral substances, for which the element content in biomass was compared with the norms of physiological requirements for humans. . In insect samples kept under conditions of stable feed supply, the content of calcium, iron, copper, zinc and manganese increased with insect growth, reaching a maximum level at the stage of the 6th moult and decreasing when the adult age was reached. At all stages of insect growth, incubation without food for 24 hours resulted in a 45% decrease in the content of copper and zinc, a 33% decrease in calcium and manganese, and a 15% decrease in iron. No effect of insect feeding schedule on the content of potassium, sodium, magnesium, and chromium, as well as lead and cadmium in biomass was revealed. . House crickets can serve a source of minerals and trace elements in the human diet, and there is a risk of excessive intake of mineral elements when using raw materials from A. domesticus in food. In spite of the fact that the results of the conducted studies indicate the possibility of adjusting the elemental composition of cricket biomass by changing the feed formulation and feeding regimes, when developing hygienic (sanitary and chemical) standards for novel objects of technical regulation - raw materials and food derived from insects, it is necessary to take into consideration the variability of mineral composition and include manganese, copper, zinc and chromium in the list of controlled indicators.
现代食品工业重新定位为生产营养密度更高的食品,除其他成分外,还富含完全蛋白质,这一趋势导致需要扩大原料基础并寻找非传统蛋白质来源,其中昆虫被认为是一种非常有前景的资源。在以前没有使用此类原料传统的国家,已批准用于食品的昆虫种类包括数千年来一直伴随人类的常见共生生物——黄粉虫(黄粉虫)、家蟋蟀(家蟋蟀)等。特别是,家蟋蟀属于多食性物种,以植物和动物底物为食,能产生自身的完全蛋白质。根据我们自己的研究结果和文献数据分析,由于矿物质成分可能含量过高,潜在用于食品目的的节肢动物的化学成分需要仔细控制。考虑到昆虫的年龄和种类归属,调节饲料成分和昆虫的喂食时间表都可被视为风险管理方法。本研究的目的是调查喂食时间表对家蟋蟀生物量中微量营养素(矿物质)成分的影响。本研究使用了ONTO LLC生产的家蟋蟀。对8个昆虫样本进行了元素分析。通过原子吸收光谱法测定昆虫生物量样本中钾、钠、钙、镁、锰、钴、铬、镍、铁、铜、锌、铅和镉的含量。从将昆虫用作相关矿物质来源的可能性角度评估了在不同喂食时间表下获得的蟋蟀生物量(第6次蜕皮期和成虫期)的元素组成,为此将生物量中的元素含量与人类生理需求标准进行了比较。在饲料供应稳定的条件下饲养的昆虫样本中,钙、铁、铜、锌和锰的含量随着昆虫生长而增加,在第6次蜕皮期达到最高水平,成年后下降。在昆虫生长的所有阶段,禁食24小时导致铜和锌含量下降45%,钙和锰含量下降33%,铁含量下降15%。未发现昆虫喂食时间表对生物量中钾、钠、镁、铬以及铅和镉的含量有影响。家蟋蟀可作为人类饮食中矿物质和微量元素的来源,使用家蟋蟀原料制作食品时存在矿物质元素摄入过量的风险。尽管所进行的研究结果表明通过改变饲料配方和喂食方式可以调节蟋蟀生物量的元素组成,但在制定新型技术法规对象——昆虫原料和衍生食品的卫生(卫生和化学)标准时,有必要考虑矿物质成分的变异性,并将锰、铜、锌和铬纳入受控指标清单。