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荨麻叶水提取物的肝脏保护作用：对生物活性和线粒体功能的见解

Nettle Leaf Water Extracts for Hepatoprotection: Insights into Bioactivity and Mitochondrial Function.

作者信息

Muceniece Ruta, Revina Beatrise Luize, Kviesis Jorens, Jansons Aris, Kopiks Kirills, Jekabsons Kaspars, Saleniece Kristine, Namniece Jana, Grigale-Sorocina Zane, Jansone Baiba

机构信息

Faculty of Medicine and Life Sciences, University of Latvia, LV-1004 Riga, Latvia.

Faculty of Science and Technology, University of Latvia, LV-1004 Riga, Latvia.

出版信息

Plants (Basel). 2025 Mar 21;14(7):992. doi: 10.3390/plants14070992.

DOI:10.3390/plants14070992
PMID:40219059
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11990370/
Abstract

This study aimed to evaluate the hepatoprotective effects of nettle ( L.) leaf water extracts on oxygen consumption in the fatty acid oxidation (FAO) pathway using an in vitro fatty liver HepG2 cell model and employing an oxygraphy approach. It also examined the impact of these extracts on HepG2 cell lipid accumulation and viability under oxidative stress. The extracts were obtained via maceration with preservatives or by sonication with/without preservatives. Their chemical composition, including polyphenols, vitamins, and minerals, was analyzed. Bioactivity was confirmed through antioxidant and antiglycation in vitro assays. The extracts contained minerals, water-soluble vitamins, and polyphenols, primarily phenolic acids and rutin. Sonication increased the polyphenol yield, advanced glycation end-product (AGE) inhibition, and total antioxidant capacity compared to maceration. The added preservatives enhanced DPPH scavenging, while SOD-mimicking effects were comparable across extraction methods. In the liver steatosis model, the nettle extracts improved HepG2 cell viability under oxidative stress, reduced lipid accumulation, and enhanced mitochondrial oxygen consumption in the FAO pathway at mitochondria complex I. These findings demonstrate the impact of nettle leaf water extracts on oxygen flux in different oxidative phosphorylation states of the FAO pathway and deepen the understanding of nettle's protective role in hepatic steatosis. The obtained results confirm the hepatoprotective effects of nettles through multiple mechanisms, primarily involving antioxidant activity, modulation of lipid accumulation, and mitochondrial protection.

摘要

本研究旨在使用体外脂肪肝HepG2细胞模型并采用氧电极法，评估荨麻叶水提取物对脂肪酸氧化（FAO）途径中氧气消耗的肝保护作用。它还研究了这些提取物在氧化应激下对HepG2细胞脂质积累和活力的影响。提取物通过添加防腐剂浸渍或添加/不添加防腐剂超声处理获得。分析了它们的化学成分，包括多酚、维生素和矿物质。通过体外抗氧化和抗糖化试验确认了生物活性。提取物含有矿物质、水溶性维生素和多酚，主要是酚酸和芦丁。与浸渍相比，超声处理提高了多酚产量、晚期糖基化终产物（AGE）抑制作用和总抗氧化能力。添加的防腐剂增强了DPPH清除能力，而超氧化物歧化酶（SOD）模拟效应在不同提取方法中相当。在肝脂肪变性模型中，荨麻提取物在氧化应激下提高了HepG2细胞活力，减少了脂质积累，并增强了FAO途径中线粒体复合物I处的线粒体氧气消耗。这些发现证明了荨麻叶水提取物对FAO途径不同氧化磷酸化状态下氧气通量产生的影响，并加深了对荨麻在肝脂肪变性中保护作用的理解。所得结果证实了荨麻通过多种机制发挥肝保护作用，主要涉及抗氧化活性、脂质积累调节和线粒体保护。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/7d7ed7682faa/plants-14-00992-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/b00b488e9b25/plants-14-00992-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/8a066c6156b0/plants-14-00992-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/7d7ed7682faa/plants-14-00992-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/b00b488e9b25/plants-14-00992-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/9daa6d62ca47/plants-14-00992-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/98ec724e8bf3/plants-14-00992-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/265ccee9d7e6/plants-14-00992-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/6522e237509c/plants-14-00992-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/1cc1689c8539/plants-14-00992-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/66adc11371cb/plants-14-00992-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/8a066c6156b0/plants-14-00992-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1152/11990370/7d7ed7682faa/plants-14-00992-g009.jpg

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ACS Omega. 2024 May 27;9(23):25094-25105. doi: 10.1021/acsomega.4c02433. eCollection 2024 Jun 11.
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Chemical Constituents of Stinging Nettle ( L.): A Comprehensive Review on Phenolic and Polyphenolic Compounds and Their Bioactivity.荨麻（L.）的化学成分：酚类和多酚类化合物及其生物活性的综合评价。
Int J Mol Sci. 2024 Mar 18;25(6):3430. doi: 10.3390/ijms25063430.
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Metabolic-associated fatty liver disease: a selective review of pathogenesis, diagnostic approaches, and therapeutic strategies.
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Front Med (Lausanne). 2024 Jan 23;11:1291501. doi: 10.3389/fmed.2024.1291501. eCollection 2024.
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Using plant-based compounds as preservatives for meat products: A review.使用植物性化合物作为肉类产品的防腐剂：综述
Heliyon. 2023 Jun 10;9(6):e17071. doi: 10.1016/j.heliyon.2023.e17071. eCollection 2023 Jun.
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New insights of the application of water or ethanol-water plant extract rich in active compounds in food.富含活性化合物的水或乙醇 - 水植物提取物在食品中的应用新见解。
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