Wolska Jolanta, Janda Katarzyna, Szkyrpan Sylwia, Gutowska Izabela
Pomeranian J Life Sci. 2015;61(3):315-8.
Stinging nettle (Urtica dioicd L.) is one of the most valuable plants used in phytotherapy. The herbal raw material is a herb (Urticae herba), leaves (Urticae folium), roots (Urticae radix) and seeds (Urticae semina). This plant is a good source of vitamins, minerals, fibre, protein and biologically active compounds with antioxidant properties. The literature provides limited information about the chemical composition and properties of the seed heads. No papers are available on the effect of extracts of this plant on catalase activity in human cells. The aim of this study was to investigate the impact of stinging nettle (Urtica dioica L.) extracts on the antioxidant activity of catalase in THP1 macrophages.
Two types of extracts: water and alcohol, at two different concentrations, were used in experiments. Nettle was collected in September and October in 2012 in the area of Szczecin. The collected plant material was frozen and lyophilized. After those procedures water and alcohol extracts of nettle were prepared and then added to THP1 cells.
The antioxidant activity of catalase was established with the spectrophotometric method. The study showed that both extracts (water and alcohol) significantly increased the antioxidant activity of catalase in THP1 cells. The increase in catalase was directly proportional to the concentration of the added alcohol extract.
荨麻（Urtica dioicd L.）是植物疗法中最具价值的植物之一。草药原料包括全草（Urticae herba）、叶子（Urticae folium）、根（Urticae radix）和种子（Urticae semina）。这种植物富含维生素、矿物质、纤维、蛋白质以及具有抗氧化特性的生物活性化合物。关于种子头的化学成分和特性，文献中提供的信息有限。目前尚无关于该植物提取物对人体细胞过氧化氢酶活性影响的研究报告。本研究的目的是探讨荨麻（Urtica dioica L.）提取物对THP1巨噬细胞中过氧化氢酶抗氧化活性的影响。
实验使用了两种不同浓度的水提取物和醇提取物。荨麻于2012年9月和10月在什切青地区采集。采集的植物材料经冷冻和冻干处理。经过这些步骤后，制备了荨麻的水提取物和醇提取物，然后将其添加到THP1细胞中。
采用分光光度法测定过氧化氢酶的抗氧化活性。研究表明，两种提取物（水提取物和醇提取物）均显著提高了THP1细胞中过氧化氢酶的抗氧化活性。过氧化氢酶活性的增加与添加的醇提取物浓度成正比。
