Zhang Shuai, Li Tianyuan, Liu Shan, Qi Xinliang, Yang Yu, Zhang Jiancheng, Jia Luting, Wang Pengfei, Mu Xiaopeng
College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, China.
Plants (Basel). 2025 Apr 2;14(7):1103. doi: 10.3390/plants14071103.
Anthocyanins play a pivotal role in determining the color diversity in the flowers and fruits of (Bge.) Sok. This study performed a metabolomic analysis of the flowers and fruits of two varieties differing in pigmentation phenotypes ('Jinou 1' and 'Nongda 5'), and the results indicated that the cyanidin, pelargonidin, paeonidin, and delphinidin were the main substances serving as the primary pigments contributing to their striking chromatic divergence between two varieties. Transcriptome profiling revealed that several key structural genes (, , , and ) in the anthocyanin biosynthesis pathway exhibited significantly elevated expression levels in 'Jinou 1' compared to 'Nongda 5'. Further metabolomic and transcriptomic correlation analyses identified that and exhibited strong positive associations with anthocyanin pathway metabolites in both floral and fruit tissues. Notably, displayed the strongest correlation with the metabolite profiles, suggesting it may serve as a core regulatory component of the anthocyanin biosynthesis. This research provides new insights into the regulatory mechanisms of anthocyanin biosynthesis in .
花色苷在决定紫斑牡丹（Paeonia rockii (Stern) Solv.）花和果实的颜色多样性方面起着关键作用。本研究对两个色素沉着表型不同的品种（‘锦锈1号’和‘农大5号’）的花和果实进行了代谢组学分析，结果表明矢车菊素、天竺葵素、芍药素和飞燕草素是导致两个品种之间显著颜色差异的主要色素物质。转录组分析显示，与‘农大5号’相比，花色苷生物合成途径中的几个关键结构基因（PAL、C4H、4CL和CHS）在‘锦锈1号’中的表达水平显著升高。进一步的代谢组学和转录组学相关性分析确定，DFR和ANS在花和果实组织中均与花色苷途径代谢物表现出强烈的正相关。值得注意的是，ANS与代谢物谱的相关性最强，表明它可能是花色苷生物合成的核心调控成分。本研究为紫斑牡丹花色苷生物合成的调控机制提供了新的见解。
