使用通过翻译抑制进行单细胞能量代谢分析（SCENITH）对活化T淋巴细胞进行代谢谱分析。

Metabolic Profiling of Activated T Lymphocytes Using Single-Cell Energetic Metabolism by Profiling Translation Inhibition (SCENITH).

作者信息

Sinning Katrin, Hochrein Sophia M, Gubert Gabriela F, Vaeth Martin

机构信息

Würzburg Institute of Systems Immunology, Max Planck Research Group, Julius-Maximilians University of Würzburg, Würzburg, Germany.

出版信息

Methods Mol Biol. 2025;2904:259-271. doi: 10.1007/978-1-0716-4414-0_18.

DOI:10.1007/978-1-0716-4414-0_18

PMID:40220239

Abstract

Metabolic reprogramming is increasingly recognized as a fundamental aspect of T cell activation, influencing the differentiation, proliferation, and effector functions of lymphocytes. Measuring and screening the metabolic states of activated T cells provide insights into the dynamic interplay between cellular metabolism and immune function. In the following chapter, we provide a simple protocol based on the publication of Argüello et al. [1] to analyze the metabolic state of activated T cells at the single-cell level using standard flow cytometry.

摘要

代谢重编程日益被认为是T细胞活化的一个基本方面，影响淋巴细胞的分化、增殖和效应功能。测量和筛选活化T细胞的代谢状态有助于深入了解细胞代谢与免疫功能之间的动态相互作用。在接下来的章节中，我们基于阿圭略等人[1]的发表成果提供一个简单的方案，以使用标准流式细胞术在单细胞水平分析活化T细胞的代谢状态。