Sinning Katrin, Hochrein Sophia M, Gubert Gabriela F, Vaeth Martin
Würzburg Institute of Systems Immunology, Max Planck Research Group, Julius-Maximilians University of Würzburg, Würzburg, Germany.
Methods Mol Biol. 2025;2904:259-271. doi: 10.1007/978-1-0716-4414-0_18.
Metabolic reprogramming is increasingly recognized as a fundamental aspect of T cell activation, influencing the differentiation, proliferation, and effector functions of lymphocytes. Measuring and screening the metabolic states of activated T cells provide insights into the dynamic interplay between cellular metabolism and immune function. In the following chapter, we provide a simple protocol based on the publication of Argüello et al. [1] to analyze the metabolic state of activated T cells at the single-cell level using standard flow cytometry.
代谢重编程日益被认为是T细胞活化的一个基本方面，影响淋巴细胞的分化、增殖和效应功能。测量和筛选活化T细胞的代谢状态有助于深入了解细胞代谢与免疫功能之间的动态相互作用。在接下来的章节中，我们基于阿圭略等人[1]的发表成果提供一个简单的方案，以使用标准流式细胞术在单细胞水平分析活化T细胞的代谢状态。
