Vlácil F, Vins I
Nahrung. 1985;29(5):467-72. doi: 10.1002/food.19850290518.
A method is described for the determination of nitrates in cow milk, human milk, milk powder or milk-based infant formulae using liquid chromatography on Spheron DEAE and a direct photometric detection (205 nm). The influence of removing proteins by precipitation with Carrez reagent on the accuracy of determination was studied. The proposed method gives identical results with the reference method (photometry after reduction of nitrate to nitrite) but is more rapid. Its limit of determination is 0.5 mg NO3-/l of milk, its reproducibility is 4% (relative standard deviation).
本文描述了一种使用Spheron DEAE进行液相色谱分析并采用直接光度检测（205nm）来测定牛奶、人乳、奶粉或以牛奶为基础的婴儿配方奶粉中硝酸盐含量的方法。研究了用Carrez试剂沉淀去除蛋白质对测定准确性的影响。所提出的方法与参考方法（将硝酸盐还原为亚硝酸盐后进行光度测定）给出的结果相同，但速度更快。其测定限为0.5mg NO3-/l牛奶，重现性为4%（相对标准偏差）。
