Tuhácková Z, Gryga P, Helmich O, Dusek Z, Hradec J
Neoplasma. 1985;32(3):323-34.
Labeled 14-methylhexadecanoic acid was administered to normal rats, animals bearing the Walker 256 tumor at various stages of its growth and to tumor-resistant rats and its distribution in lipids (free fatty acids, triglycerides, phospholipids and cholesteryl esters) was studied during 15 min to 3 hours following its injection in the liver, blood and tumor tissue. The period of the most active tumor growth was associated with a significantly better utilization of this fatty acid for the synthesis of lipids. The quantities of radioactive triglycerides, phospholipids and cholesteryl ester in the liver were highly increased during this time. In tumor-resistant animals the levels of radioactive lipids were similar to those in control normal animals but the turn-over of 14-methylhexadecanoic acid was considerably slower than in controls. The turn-over of the cholesteryl 14-methylhexadecanoate in the liver was significantly changed at the late stages of the tumor growth. The level of this cholesteryl ester in the blood decreased progressively during the tumor growth whereas that of triglycerides increased. No significant changes were associated with the free fatty acid and phospholipids in the blood. The total radioactivity present in the tumor increased from 0.05% up to nearly 1% of the administered 14-methylhexadecanoic acid during the growth of the Walker 256 tumor. The level of radioactive cholesteryl 14-methylhexadecanoate in the tumor tissue increased progressively during 3 hours following the injection of 14-methylhexadecanoic acid. Thus the tumor growth is apparently accompanied by significant changes in the metabolism of 14-methylhexadecanoic acid and results in an enhanced synthesis of lipids in the liver tissue. The newly synthesized lipids, in particular cholesteryl 14-methylhexadecanoate, are transported in the blood stream into the tumor where they accumulate.
给正常大鼠、处于沃克256肿瘤生长各阶段的荷瘤动物以及肿瘤抗性大鼠注射标记的14-甲基十六烷酸，并在注射后15分钟至3小时内研究其在脂质（游离脂肪酸、甘油三酯、磷脂和胆固醇酯）中的分布，涉及肝脏、血液和肿瘤组织。肿瘤生长最活跃的时期与该脂肪酸用于脂质合成的利用率显著提高有关。在此期间，肝脏中放射性甘油三酯、磷脂和胆固醇酯的含量大幅增加。在肿瘤抗性动物中，放射性脂质的水平与对照正常动物相似，但14-甲基十六烷酸的周转比对照动物慢得多。在肿瘤生长后期，肝脏中14-甲基十六烷酸胆固醇酯的周转发生了显著变化。在肿瘤生长过程中，血液中这种胆固醇酯的水平逐渐下降，而甘油三酯的水平则升高。血液中的游离脂肪酸和磷脂没有显著变化。在沃克256肿瘤生长过程中，肿瘤中的总放射性从给药的14-甲基十六烷酸的0.05%增加到近1%。注射14-甲基十六烷酸后3小时内，肿瘤组织中放射性14-甲基十六烷酸胆固醇酯的水平逐渐升高。因此，肿瘤生长显然伴随着14-甲基十六烷酸代谢的显著变化，并导致肝脏组织中脂质合成增加。新合成的脂质，特别是14-甲基十六烷酸胆固醇酯，通过血流运输到肿瘤中并在那里积累。
