Delorme P, Hevor T K
Neuropathol Appl Neurobiol. 1985 Mar-Apr;11(2):117-28. doi: 10.1111/j.1365-2990.1985.tb00009.x.
Rats and mice were submitted either to the convulsant methionine sulfoximine (MSO) alone or to MSO combined with actinomycin D or methionine respectively. Twenty-four hours after the intraperitoneal administration of these compounds, the animals were killed and tissue samples were prepared for electron microscopy. Methionine sulfoximine induced 'grand mal' type seizures which were abolished by methionine. In saline controls, glycogen was as beta particles located in the cytoplasm of astrocytes, i.e. in perikarya and processes. Liver glycogen was as perinuclear masses of alpha and beta particles or as alpha particles scattered in all the cytoplasm. When the rodents were treated with MSO, glycogen was as alpha and beta particles which invaded all areas of the astrocyte cytoplasm, this increase being tremendous in perivascular end feet. Actinomycin D slowed down the accumulation of glycogen particles while methionine completely abolished it. In any case, glycogen particles were confined to the astrocytes and were never seen in other types of cells. In liver, MSO induced an important decrease or a complete disappearance of glycogen particles. When the convulsant was combined with actinomycin D or with methionine, the figures looked like those of controls. These results have been discussed in relation to the mechanism of glycogenesis in central nervous system of rodents submitted to MSO.
将大鼠和小鼠单独给予惊厥剂蛋氨酸亚砜亚胺（MSO），或分别将MSO与放线菌素D或蛋氨酸联合给予。腹腔注射这些化合物24小时后，处死动物并制备组织样本用于电子显微镜检查。蛋氨酸亚砜亚胺诱发“大发作”型癫痫发作，蛋氨酸可消除这种发作。在生理盐水对照组中，糖原呈β颗粒，位于星形胶质细胞的细胞质中，即在胞体和突起中。肝糖原呈核周α和β颗粒团块或α颗粒散布于整个细胞质中。当用MSO处理啮齿动物时，糖原呈α和β颗粒，侵入星形胶质细胞细胞质的所有区域，这种增加在血管周围终足中极为显著。放线菌素D减缓了糖原颗粒的积累，而蛋氨酸则完全消除了这种积累。无论如何，糖原颗粒局限于星形胶质细胞，在其他类型的细胞中从未见过。在肝脏中，MSO导致糖原颗粒显著减少或完全消失。当惊厥剂与放线菌素D或蛋氨酸联合使用时，结果与对照组相似。已结合接受MSO处理的啮齿动物中枢神经系统中糖原合成的机制对这些结果进行了讨论。
