Vargas-Arana Gabriel, Torres-Benítez Alfredo, Ortega-Valencia José Erick, Merino-Zegarra Claudia, Carranza-Rosales Pilar, Simirgiotis Mario J
Laboratorio de Química de Productos Naturales, Instituto de Investigaciones de la Amazonía Peruana, Avenue Abelardo Quiñones Km 2.5, Iquitos 16001, Peru.
Facultad de Industrias Alimentarias, Universidad Nacional de la Amazonía Peruana, Iquitos 16001, Peru.
Antioxidants (Basel). 2025 Feb 20;14(3):246. doi: 10.3390/antiox14030246.
is a plant of great value in traditional medicine known for its content of bioactive compounds, such as physalins and withanolides, which possess diverse biological activities. In this study, the chemical profile, antioxidant activity, and enzyme inhibition capacity of aqueous and ethanolic extracts obtained from the root, stem, leaves, calyx, and fruits of collected in Peru were evaluated. A total of forty-two compounds were detected in the extracts using UHPLC-ESI-QTOF-MS analysis. In vitro analyses revealed that leaf extracts contained the highest concentration of phenolic compounds, while leaf and fruit extracts showed the best results in FRAP, DPPH, and ABTS antioxidant tests; on the other hand, inhibition of AChE, BChE, α-glucosidase, and α-amylase enzymes was variable, but calyx and fruit extracts showed higher effectiveness. In silico analyses indicated that the compounds physagulin A, physagulin F, physagulide P, physalin B, and withaminimin showed stable interactions and favorable binding affinities with the catalytic sites of the enzymes studied. These results confirm the pharmacological potential of extracts and compounds derived from different organs of , suggesting their promising use in treating diseases related to the central nervous system and metabolic syndrome.
是一种在传统医学中具有重要价值的植物，因其含有多种生物活性化合物，如酸浆苦素和睡茄内酯，这些化合物具有多种生物活性。在本研究中，对从秘鲁采集的该植物的根、茎、叶、花萼和果实中获得的水提取物和乙醇提取物的化学特征、抗氧化活性和酶抑制能力进行了评估。使用超高效液相色谱-电喷雾电离-四极杆飞行时间质谱分析在提取物中总共检测到42种化合物。体外分析表明，叶提取物中酚类化合物的浓度最高，而叶和果实提取物在铁还原抗氧化能力（FRAP）、二苯基苦味酰基自由基（DPPH）和2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）抗氧化试验中表现出最佳结果；另一方面，对乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）、α-葡萄糖苷酶和α-淀粉酶的抑制作用各不相同，但花萼和果实提取物显示出更高的有效性。计算机模拟分析表明，酸浆苦素A、酸浆苦素F、酸浆苦素P、酸浆苦素B和睡茄胺表现出与所研究酶的催化位点稳定的相互作用和良好的结合亲和力。这些结果证实了该植物不同器官提取物和化合物的药理潜力，表明它们在治疗与中枢神经系统和代谢综合征相关疾病方面具有广阔的应用前景。
