Mao Ying, Liu Xinmei, Xin Leiting, Cai Pei, Lu Wangyang, Chen Wenxing
National Engineering Lab for Textile Fiber Materials & Processing Technology, Zhejiang Sci-Tech University, Hangzhou 310018, China; Zhejiang Provincial Innovation Center of Advanced Textile Technology, Shaoxing 312000, China; Zhejiang Sci-Tech University Shengzhou Innovation Research Institute, Shengzhou 312451, China.
National Engineering Lab for Textile Fiber Materials & Processing Technology, Zhejiang Sci-Tech University, Hangzhou 310018, China; Zhejiang Provincial Innovation Center of Advanced Textile Technology, Shaoxing 312000, China; Zhejiang Sci-Tech University Shengzhou Innovation Research Institute, Shengzhou 312451, China.
Int J Biol Macromol. 2025 May;309(Pt 4):143130. doi: 10.1016/j.ijbiomac.2025.143130. Epub 2025 Apr 12.
Bacterial infection remains a thorny problem in food, medicine, and other fields. Due to the emergence of antibiotic-resistant bacteria, it is critical to develop an effective strategy to yield novel antibacterial agents. Herein, a new type of non-antibiotic antimicrobial material was successfully synthesized by grafting ε-polylysine (EPL) onto silk fibroin (SF). The resulting ε-polylysine-modified silk fibroin (SF-EPL) possessed the possibility to be processed into different formats. As the EPL content increased, SF-EPL exhibited higher positive charge levels, similar to those of EPL. Notably, the incorporation of 10 % EPL endowed superior antibacterial effects against E. coli and S. aureus (>90 %), while maintaining excellent biocompatibility. Furthermore, SF-10%EPL effectively extended the shelf life of cherry tomatoes, significantly delaying weight loss and the decline in titratable acidity content. The obtained SF-EPL may represent a promising substitute for food preservation and biomedical applications.
细菌感染在食品、医药和其他领域仍然是一个棘手的问题。由于抗生素耐药菌的出现，制定有效的策略以生产新型抗菌剂至关重要。在此，通过将ε-聚赖氨酸（EPL）接枝到丝素蛋白（SF）上，成功合成了一种新型非抗生素抗菌材料。所得的ε-聚赖氨酸修饰丝素蛋白（SF-EPL）具有加工成不同形式的可能性。随着EPL含量的增加，SF-EPL表现出更高的正电荷水平，与EPL相似。值得注意的是，掺入10%的EPL对大肠杆菌和金黄色葡萄球菌具有优异的抗菌效果（>90%），同时保持了良好的生物相容性。此外，SF-10%EPL有效地延长了樱桃番茄的货架期，显著延缓了重量损失和可滴定酸度含量的下降。所获得的SF-EPL可能是食品保鲜和生物医学应用的一种有前途的替代品。
