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生物素缺乏的构巢曲霉中的生长、葡萄糖代谢和黑色素形成

Growth, glucose metabolism and melanin formation in biotin-deficient Aspergillus nidulans.

作者信息

Desai J D, Modi V V

出版信息

Folia Microbiol (Praha). 1977;22(1):55-60. doi: 10.1007/BF02876995.

Abstract

Biotin deficiency resulted in an increased growth rate of Aspergillus nidulans. The activities of hexokinase and aldolase were not much changed during the growth cycle, but activities of glucose-6-phosphate dehydrogenase and NADP-linked glutamate dehydrogenase increased significantly during the exponential phase. This change was remarkable during biotin deficiency. In contrast to the higher growth rate and respiration rate during biotin deficiency the activities of NAD(P)H oxidoreductases were low. An inverse relationship between the activity of tyrosinase and melanin content was observed. A role of the DOPA-DOPA-quinone system in maintaining culture growth is suggested.

摘要

生物素缺乏导致构巢曲霉生长速率增加。己糖激酶和醛缩酶的活性在生长周期中变化不大,但葡萄糖-6-磷酸脱氢酶和NADP连接的谷氨酸脱氢酶的活性在指数生长期显著增加。这种变化在生物素缺乏时尤为明显。与生物素缺乏时较高的生长速率和呼吸速率相反,NAD(P)H氧化还原酶的活性较低。观察到酪氨酸酶活性与黑色素含量之间呈负相关。提示了多巴-多巴醌系统在维持培养物生长中的作用。

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