Arst H N, Parbtani A A, Cove D J
Mol Gen Genet. 1975;138(2):164-71.
A mutation leading to partial loss of NAD-linked ("catabolic') glutamate dehydrogenase does not affect the regulation of ammonium-repressible activities in Aspergillus nidulans. This mutation has been used to show that NAD-linked glutamate dehydrogenase does not normally participate in ammonium assimilation. A mutation leading to loss of NADP-linked ("anabolic') glutamate dehydrogenase has been used to show that NADP-linked glutamate dehydrogenase is not normally involved in glutamate catabolism. Strains defective in either enzyme are useful for determining which amino acids are metabolised via transamination to yield glutamate rather than via deamination to yield ammonium.
导致NAD连接的（“分解代谢的”）谷氨酸脱氢酶部分缺失的突变并不影响构巢曲霉中铵抑制活性的调节。该突变已被用于表明NAD连接的谷氨酸脱氢酶通常不参与铵同化。导致NADP连接的（“合成代谢的”）谷氨酸脱氢酶缺失的突变已被用于表明NADP连接的谷氨酸脱氢酶通常不参与谷氨酸分解代谢。这两种酶有缺陷的菌株可用于确定哪些氨基酸是通过转氨作用代谢产生谷氨酸，而不是通过脱氨作用产生铵。
