Hervé Flore M, Borras Eva, Gibson Patrick, McCartney Mitchell M, Kenyon Nicholas J, Davis Cristina E
Mechanical and Aerospace Engineering, UC Davis, Davis, CA, United States of America.
UC Davis Lung Center, Davis, CA, United States of America.
J Breath Res. 2025 May 6;19(3). doi: 10.1088/1752-7163/adccef.
Human skin is an important source of volatile organic compounds (VOCs) offering noninvasive methods to gain clinical metabolite information. This work was focused on the development of a skin sampling device based on a dynamic headspace sampling method with the addition of temperature to increase VOC metabolite recovery. The device preconcentrates skin VOC emissions onto a sorbent substrate, which can either be preserved for offline analysis or attached to a real time sensor downstream. In this work, skin VOC samples were analyzed offline using thermal desorption-gas chromatography-mass spectrometry. A list of 10 common skin VOCs was pre-selected to optimize parameters of sampling time, sampling temperature, and sorbent selection. Overall, this study highlights an effective skin VOC sampling technology with a heating dimension (40 °C, rather than 30 °C or no heating) with a sampling time of 15 min (rather than 5 or 30 mins) and onto Tenax TA sorbent (rather than PDMS), which collectively increases the recovery of compounds with lower vapor pressure and decreases the observed variability in skin VOC measurements. Finally, a list of 79 skin VOC compounds were detected and identified within a cohort of 20 young, healthy volunteers.
人体皮肤是挥发性有机化合物（VOCs）的重要来源，为获取临床代谢物信息提供了非侵入性方法。这项工作的重点是开发一种基于动态顶空采样方法的皮肤采样装置，并增加温度以提高VOC代谢物的回收率。该装置将皮肤VOC排放物预浓缩到吸附剂基质上，该基质既可以保存用于离线分析，也可以连接到下游的实时传感器上。在这项工作中，使用热解吸-气相色谱-质谱联用仪对皮肤VOC样品进行离线分析。预先选择了10种常见的皮肤VOCs清单，以优化采样时间、采样温度和吸附剂选择等参数。总体而言，本研究突出了一种有效的皮肤VOC采样技术，加热温度为40°C（而非30°C或不加热），采样时间为15分钟（而非5分钟或30分钟），吸附剂为Tenax TA（而非PDMS），这些因素共同提高了低蒸气压化合物的回收率，并降低了皮肤VOC测量中观察到的变异性。最后，在20名年轻健康志愿者组成的队列中检测并鉴定出了79种皮肤VOC化合物。
