高良姜素外泌体通过miR-10b/P53轴诱导胃癌细胞凋亡。

Galangin exosomes induce cell apoptosis through miR-10b/P53 axis in gastric cancer.

作者信息

Li Qirong, Zhang Yongsheng, Shi Bohe, Lin Chao, Feng Qiang, Zhou Hengzong, Hao Ming, Ding Yu, Ma Chaoyang, Mu Jianfeng, Wang Dongxu

机构信息

Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, China.

Outpatient Department of Pediatrics, Children's Medical Center, The First Hospital of Jilin University, Changchun, China.

出版信息

Sci Rep. 2025 Apr 15;15(1):12876. doi: 10.1038/s41598-025-95908-9.

DOI:10.1038/s41598-025-95908-9

PMID:40234641

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12000617/

Abstract

Galangin is an extract of traditional Chinese medicine with anti-cancer effect. Exosomes (Exo) contain RNA, peptides, or proteins and communicate between cells through a bio-genesis pathway. In the present study, Gastric cancer (GC) cell exosomes containing galangin were obtained. MicroRNAs (miRNAs) in exosomes containing galangin (galangin-Exo) were screened by RNA-seq. GC cells were treated with galangin-Exo and the changes of cell proliferation were analyzed. Besides, the mouse model of GC was established to verify the effect of galangin-Exo in vivo. The results showed that galangin-Exo inhibited the growth of AGS and BGC823 cells. Additionally, RNA-seq data showed that miR-10b-5p was differentially expressed in exosomes. The reduced expression of miR-10b-5p which increased the expression of P53 inhibited the growth and induced apoptosis of GC cells. The in vivo results confirmed that exosomes can inhibit tumor growth by delivering galangin. In conclusion, our results suggest that galangin-Exo have anti-cancer effect on GC and can be used as a potential therapeutic strategy.

摘要

高良姜素是一种具有抗癌作用的中药提取物。外泌体（Exo）含有RNA、肽或蛋白质，并通过生物发生途径在细胞间进行通讯。在本研究中，获得了含有高良姜素的胃癌（GC）细胞外泌体。通过RNA测序筛选了含有高良姜素的外泌体（高良姜素-Exo）中的微小RNA（miRNA）。用高良姜素-Exo处理GC细胞，并分析细胞增殖的变化。此外，建立了GC小鼠模型以验证高良姜素-Exo在体内的作用。结果表明，高良姜素-Exo抑制了AGS和BGC823细胞的生长。此外，RNA测序数据显示miR-10b-5p在外泌体中差异表达。miR-10b-5p表达降低，增加了P53的表达，抑制了GC细胞的生长并诱导其凋亡。体内结果证实外泌体可通过递送高良姜素抑制肿瘤生长。总之，我们的结果表明高良姜素-Exo对GC具有抗癌作用，可作为一种潜在的治疗策略。