Cameirão Cristina, Pereira José Alberto, Tavares Rui, Lino-Neto Teresa, Baptista Paula
CIMO, LA SusTEC, Instituto Politécnico de Bragança, Campus de Santa Apolónia 5300-253 Bragança, Portugal.
Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Campus de Gualtar 4710-057 Braga, Portugal.
Curr Res Insect Sci. 2025 Mar 26;7:100110. doi: 10.1016/j.cris.2025.100110. eCollection 2025.
In nature, plants and insects engage in intricate interactions. Despite the increasing knowledge of the microbiomes of plants and insects, the extent to which they exchange and alter each other's microbiomes remains unclear. In this work, the bacterial community associated with nymphs of (Hemiptera: Aphrophoridae), the stems of where the nymphs were feeding, and the foam produced by the nymphs, were studied by culture-dependent and -independent approaches, with an attempt to elucidate the exchange of bacteria between plants and insects. The results suggest that both approaches complement each other, as many bacterial genera identified by metabarcoding were not detected by culturing, and vice versa. Overall, stems and foam exhibited higher bacterial diversity than nymphs, with all the samples showing enrichment in bacteria known to provide diverse benefits to their host. Stems and foam were the most similar in bacterial composition, but and dominated the stems, whereas and dominated the foam. Nymphs exhibit the most distinct bacterial composition, yet more similar to that found in the stem compared to the foam. Indeed, nymphs were enriched on endosymbiotic bacteria, mostly Sulcia and , not found in the stem and foam. Nevertheless, during feeding, nymphs appeared to exchange several bacteria genera with , with a significant number being incorporated into the bacteriome of the nymph. The genera and are likely the most exchanged. Nymphs also appear to exchange bacteria to the foam, notably species from the and genera. More studies to infer the functions of the shared bacteria between - are needed.
在自然界中，植物和昆虫有着复杂的相互作用。尽管人们对植物和昆虫的微生物群的了解日益增加，但它们相互交换和改变彼此微生物群的程度仍不清楚。在这项研究中，采用依赖培养和不依赖培养的方法，对与（半翅目：沫蝉科）若虫相关的细菌群落、若虫取食的植物茎干以及若虫产生的泡沫进行了研究，试图阐明植物和昆虫之间细菌的交换情况。结果表明，这两种方法相互补充，因为通过宏条形码鉴定出的许多细菌属在培养过程中未被检测到，反之亦然。总体而言，茎干和泡沫的细菌多样性高于若虫，所有样本中已知对宿主有多种益处的细菌都有所富集。茎干和泡沫的细菌组成最为相似，但[具体细菌属1]和[具体细菌属2]在茎干中占主导地位，而[具体细菌属3]和[具体细菌属4]在泡沫中占主导地位。若虫的细菌组成最为独特，但与茎干中的细菌组成相比，比与泡沫中的更为相似。事实上，若虫富含内共生细菌，主要是[具体细菌属5]、苏尔西亚菌（Sulcia）和[具体细菌属6]，这些细菌在茎干和泡沫中未被发现。然而，在取食过程中，若虫似乎与[植物名称]交换了几个细菌属，其中有大量细菌被纳入若虫的细菌群落。[具体细菌属7]和[具体细菌属8]可能是交换最多的属。若虫似乎还将细菌交换到泡沫中，特别是来自[具体细菌属9]和[具体细菌属10]的物种。需要更多的研究来推断[植物名称]和若虫之间共享细菌的功能。
