Knobloch J
Trop Med Parasitol. 1985 Jun;36(2):91-3.
Four immunoassays (IgG ELISA, indirect haemagglutination = IHA, IgM ELISA and antigen ELISA) for determination of humoral antibody and of circulating antigen, respectively, were applied to sera of 45 Peruvian fascioliasis patients who were identified by demonstration of Fasciola eggs in faeces. All of the sera were reactive by at least one of the assays. Sensitivity rates were 0.98 for IgG ELISA, 0.56 for IHA, 0.51 for IgM ELISA and 0.20 for antigen ELISA. Among those 34 patients who were followed up after praziquantel therapy there was a different pattern of immunoassay results between patients who stopped excreting eggs after therapy and those who continued to pass eggs. Cure was indicated by disappearance of both IgM antibody and antigen levels during three months after treatment, whereas persistence of infection corresponded to new or re-occurrence of IgM antibody and/or circulating antigen during the period of observation. Since there was no apparent relation of immunoassay results to therapy, it is presumed that the Peruvian patients did not respond to praziquantel at all.
分别采用四种免疫测定法（IgG酶联免疫吸附测定、间接血凝反应 = IHA、IgM酶联免疫吸附测定和抗原酶联免疫吸附测定）来检测体液抗体和循环抗原，这些测定法应用于45名秘鲁片形吸虫病患者的血清，这些患者通过粪便中发现片形吸虫卵得以确诊。所有血清至少通过一种测定法呈现反应性。IgG酶联免疫吸附测定的灵敏度为0.98，IHA为0.56，IgM酶联免疫吸附测定为0.51，抗原酶联免疫吸附测定为0.20。在34名接受吡喹酮治疗后进行随访的患者中，治疗后停止排虫卵的患者和继续排虫卵的患者之间免疫测定结果模式不同。治疗后三个月内IgM抗体和抗原水平消失表明治愈，而在观察期内IgM抗体和/或循环抗原重新出现或再次出现则表明感染持续存在。由于免疫测定结果与治疗之间没有明显关系，因此推测秘鲁患者对吡喹酮根本没有反应。
