来自VITGV38菌株（MCC 4869）的ψ，ψ-胡萝卜素的生物合成途径。

Biosynthetic Pathway of psi, psi-Carotene from sp. VITGV38 (MCC 4869).

作者信息

Pattapulavar Veilumuthu, Ramanujam Sathiyabama, Sekaran Manoj, Chandrasekaran Rajasekaran, Panchal Shweta, Christopher John Godwin

机构信息

Department of Biomedical Sciences, School of BioSciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.

Department of Science and Humanities, Karpagam Academy of Higher Education, Coimbatore, India.

出版信息

Front Microbiol. 2025 Apr 1;16:1548894. doi: 10.3389/fmicb.2025.1548894. eCollection 2025.

DOI:10.3389/fmicb.2025.1548894

PMID:40236476

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11998277/

Abstract

INTRODUCTION

Endophytic play a crucial role in plant-microbe interactions, often exhibiting beneficial biological activities, including the production of bioactive secondary metabolites. This study aimed to characterize the carotene biosynthetic pathway of a newly discovered sp. VITGV38, isolated from tomato (Lyc).

METHODS

The strain ( sp. VITGV38, MCC4869) was cultured in starch casein broth, and its metabolite profile was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). Whole-genome sequencing was performed using the Illumina platform, and the biosynthetic gene clusters (BGCs) were identified using antiSMASH.

RESULTS

Metabolite analysis revealed the presence of pigmented compounds, including psi, psi-carotene, detected at a retention time of 25.094, constituting 1.26% of the crude extract. Whole-genome sequencing uncovered an 8.27 Mb genome encoding 26 distinct secondary metabolite biosynthetic gene clusters. Notably, scaffold 26.3 was identified as a terpene biosynthetic cluster, accounting for 62% of the total secondary metabolite content and associated with carotenoid and β-carotene production.

DISCUSSION

These findings highlight the biotechnological potential of sp. VITGV38 for sustainable microbial production of carotenoids, offering an eco-friendly alternative to synthetic pigments. This study provides valuable insights into microbial carotenoid biosynthesis and its potential industrial applications.

摘要

引言

内生菌在植物 - 微生物相互作用中起着关键作用，通常表现出有益的生物活性，包括产生生物活性次生代谢产物。本研究旨在表征从番茄（Lyc）中分离出的新发现的[具体菌种名称未给出]VITGV38的类胡萝卜素生物合成途径。

方法

该菌株（[具体菌种名称未给出]VITGV38，MCC4869）在淀粉酪蛋白肉汤中培养，其代谢产物谱通过气相色谱 - 质谱联用（GC - MS）进行分析。使用Illumina平台进行全基因组测序，并使用antiSMASH鉴定生物合成基因簇（BGCs）。

结果

代谢产物分析显示存在色素化合物，包括在保留时间为25.094时检测到的ψ，ψ - 胡萝卜素，占粗提物的1.26％。全基因组测序揭示了一个8.27 Mb的基因组，编码26个不同的次生代谢产物生物合成基因簇。值得注意的是，支架26.3被鉴定为萜烯生物合成簇，占次生代谢产物总量的62％，并与类胡萝卜素和β - 胡萝卜素的产生相关。