Xu Yuan, On Thomas J, Preul Mark C
The Loyal and Edith Davis Neurosurgical Research Laboratory, Barrow Neurological Institute, St. Joseph's Hospital and Medical Center, Phoenix, AZ, United States.
Front Surg. 2025 Apr 1;12:1559195. doi: 10.3389/fsurg.2025.1559195. eCollection 2025.
Transcranial low-intensity focused ultrasound (FUS) enables noninvasive, targeted, and reversible blood-brain barrier (BBB) disruption, facilitating drug delivery and liquid biopsy of the brain. Using fluorescein sodium (FNa) with macroscopic widefield fluorescence and microscopic confocal laser endomicroscopy (CLE) imaging, we assessed BBB permeability after applying a frameless, electromagnetic-guided FUS system in a porcine model and confirmed with established MRI protocol and conventional histology. Both macroscopic and microscopic FNa fluorescence imaging findings correlated with contrast-enhanced MRI, providing direct evidence of BBB disruption. This approach demonstrates the utility of FNa for evaluating BBB permeability in preclinical studies.
经颅低强度聚焦超声（FUS）能够实现无创、靶向且可逆的血脑屏障（BBB）破坏，促进脑部药物递送和液体活检。通过使用具有宏观宽视野荧光和微观共聚焦激光内镜检查（CLE）成像的荧光素钠（FNa），我们在猪模型中应用无框架电磁引导FUS系统后评估了血脑屏障通透性，并通过既定的MRI方案和传统组织学进行了确认。宏观和微观FNa荧光成像结果均与对比增强MRI相关，为血脑屏障破坏提供了直接证据。这种方法证明了FNa在临床前研究中评估血脑屏障通透性的实用性。
