Bode C, Matsueda G R, Hui K Y, Haber E
Science. 1985 Aug 23;229(4715):765-7. doi: 10.1126/science.4023710.
A specific fibrinolytic agent was synthesized by covalently coupling urokinase to a monoclonal antibody that was fibrin-specific and did not cross-react with fibrinogen. The antibody was raised against a synthetic peptide representing the seven amino-terminal residues of the beta chain of human fibrin. The urokinase-antifibrin conjugate retained the original binding specificity of the antibody and showed 100-fold increased fibrinolysis in vitro when compared to unmodified urokinase. The presence of human fibrinogen at plasma concentration did not influence these properties.
通过将尿激酶与一种纤维蛋白特异性且不与纤维蛋白原交叉反应的单克隆抗体共价偶联，合成了一种特异性纤溶酶原激活剂。该抗体是针对代表人纤维蛋白β链七个氨基末端残基的合成肽产生的。与未修饰的尿激酶相比，尿激酶 - 抗纤维蛋白缀合物保留了抗体原有的结合特异性，并且在体外显示出纤溶活性提高了100倍。血浆浓度的人纤维蛋白原的存在不影响这些特性。
