Li Meng, Fu Ying, Liang Xinyue, Liu Xueting, Huang Li, Zhang Sen, Zhang Wei, Tang Kexuan, Shen Qian
School of Agriculture and Biology, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Minhang, Shanghai, China.
Plant Cell Environ. 2025 Aug;48(8):5789-5801. doi: 10.1111/pce.15560. Epub 2025 Apr 16.
Trichomes serve as the first line of defense for plants against biotic and abiotic stress. The HD-ZIP IV family plays conserved roles in regulating the development of trichomes in different species, but its specific regulatory mechanism in tomato is not fully understood. In this study, we demonstrate that SlHDZIV3 and SlHDZIV9 are positive regulators of trichome development in tomato, and they function in a partially redundant yet distinct manner. SlHDZIV3 primarily affects the formation of long trichomes on tomato leaves and early formed leaf petiole, while SlHDZIV9 influence the formation of long trichomes across a broader range of tissues, including the fruit trichomes. The CRISPR/Cas9 mutants of SlHDZIV9 exhibit a reduction in fruit trichomes, leading to an extended postharvest shelf-life of tomato fruit. In addition, both SlHDZIV3 and SlHDZIV9 are essential for jasmonic acid-mediated regulation of long trichomes formation. This study enhances our understanding of trichome initiation and development in tomato and provides new strategies for improving postharvest preservation of tomato fruits.
腺毛是植物抵御生物和非生物胁迫的第一道防线。HD-ZIP IV家族在调控不同物种腺毛发育方面发挥着保守作用，但其在番茄中的具体调控机制尚不完全清楚。在本研究中，我们证明SlHDZIV3和SlHDZIV9是番茄腺毛发育的正向调节因子，它们以部分冗余但又不同的方式发挥作用。SlHDZIV3主要影响番茄叶片和早期形成的叶柄上长腺毛的形成，而SlHDZIV9影响更广泛组织中长腺毛的形成，包括果实腺毛。SlHDZIV9的CRISPR/Cas9突变体果实腺毛减少，导致番茄果实采后货架期延长。此外，SlHDZIV3和SlHDZIV9对于茉莉酸介导的长腺毛形成调控都是必不可少的。本研究增进了我们对番茄腺毛起始和发育的理解，并为改善番茄果实采后保鲜提供了新策略。
