一种靶向严重急性呼吸综合征冠状病毒2（SARS-CoV-2）N蛋白的重组杆状病毒的构建及免疫原性分析
Construction and immunogenicity analysis of a recombinant baculovirus targeting the N protein of SARS-CoV-2.
作者信息
Yu Bohan, Mo Xiaoli, Sui Li, Fang Yujia, Tang Xudong, Qian Ping
机构信息
Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.
Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang, 212100, China.
出版信息
Biotechnol Lett. 2025 Apr 16;47(3):42. doi: 10.1007/s10529-025-03576-6.
OBJECTIVES
This study aimed to construct recombinant baculoviruses capable of expressing the nucleocapsid N protein of SARS-CoV-2 and to assess the effects of co-administration with sodium butyrate on its expression and immunogenicity.
RESULTS
The recombinant BmNPV expressed green fluorescent protein in BmN cells and N protein in mammalian cells. The addition of sodium butyrate significantly enhanced the expression of N protein in HEK293T cells. Following intramuscular injection of recombinant BmNPV into mice, specific antibodies against the N protein were detectedon day 7, 21, and 35. Co-administration with sodium butyrate (1000 mg/kg body weight) significantly enhanced the immunogenicity of the recombinant virus.
CONCLUSIONS
Recombinant BmNPV expressing the SARS-CoV2 N protein successfully inducedthe anti-N immunogenicity in mice, providing a solid foundationfor the development of novel subunit vaccines against SARS-CoV-2.
目的
本研究旨在构建能够表达严重急性呼吸综合征冠状病毒2（SARS-CoV-2）核衣壳N蛋白的重组杆状病毒，并评估与丁酸钠共同给药对其表达和免疫原性的影响。
结果
重组BmNPV在BmN细胞中表达绿色荧光蛋白，在哺乳动物细胞中表达N蛋白。丁酸钠的添加显著增强了HEK293T细胞中N蛋白的表达。将重组BmNPV肌肉注射到小鼠体内后，在第7天、21天和35天检测到针对N蛋白的特异性抗体。与丁酸钠（1000mg/kg体重）共同给药显著增强了重组病毒的免疫原性。
结论
表达SARS-CoV-2 N蛋白的重组BmNPV成功诱导了小鼠的抗N免疫原性，为开发针对SARS-CoV-2的新型亚单位疫苗奠定了坚实基础。
Zotero 插件