Upatham E S, Kruatrachue M, Viyanant V, Khunborivan V, Kunatham L
Southeast Asian J Trop Med Public Health. 1985 Mar;16(1):1-9.
Studies on the bionomics and host-parasite relationship of Robertsiella kaporensis and Malaysian Schistosoma were investigated. The study was divided into 4 parts: cultivation of snails, R. kaporensis, and maintenance of Malaysian Schistosoma life cycle, daily cercarial shedding cycle in R. kaporensis, miracidial load and cercarial shedding pattern and the infectivity of Malaysian Schistosoma cercariae. R. kaporensis were cultured in the laboratory with the use of plastic container provided with fine sand. The snails were fed with diatoms and Saraca leaves. The development period for the snail eggs was about 20-30 days, the young grew to maturity in 14-15 weeks, and the average growth rate of snails was 0.23 mm per week. The daily cercarial shedding cycle of snails had shown that the peak emergence of cercariae of Malaysian Schistosoma occurred at night, between 6-10 pm. The miracidial load which yielded the best results in terms of percentage infection rates of snails and cercarial output was the miracidial concentration of 8 miracidia per snail. The study on infectivity of Malaysian Schistosoma cercariae has shown that there was a decrease in infectivity of the cercariae to mammalian hosts as the cercariae increased in age. The percentage infection rate of mice and numbers of worms recovered were highest in mice infected with cercariae of 0-1/2 hr. old. Infection of cercariae fell rapidly after the cercariae were 16 hr. old.
对卡波罗伯茨吸虫和马来西亚血吸虫的生物学特性及宿主 - 寄生虫关系进行了研究。该研究分为4个部分：蜗牛的培养、卡波罗伯茨吸虫的培养以及马来西亚血吸虫生命周期的维持、卡波罗伯茨吸虫每日尾蚴逸出周期、毛蚴负荷和尾蚴逸出模式以及马来西亚血吸虫尾蚴的感染性。在实验室中使用装有细沙的塑料容器培养卡波罗伯茨吸虫。给蜗牛喂食硅藻和无忧花树叶。蜗牛卵的发育期约为20 - 30天，幼体在14 - 15周内发育成熟，蜗牛的平均生长速度为每周0.23毫米。蜗牛的每日尾蚴逸出周期表明，马来西亚血吸虫尾蚴的最高逸出高峰出现在夜间，即下午6点至10点之间。就蜗牛的感染率百分比和尾蚴产量而言，产生最佳结果的毛蚴负荷是每只蜗牛8个毛蚴的毛蚴浓度。对马来西亚血吸虫尾蚴感染性的研究表明，随着尾蚴年龄的增加，其对哺乳动物宿主的感染性降低。感染0 - 1/2小时龄尾蚴的小鼠的感染率百分比和回收的虫数最高。尾蚴在16小时龄后感染率迅速下降。
