Dharmkrong-at A, Sirisinha S
Southeast Asian J Trop Med Public Health. 1985 Mar;16(1):31-40.
Attempts were made to induce acquired immunity against Angiostrongylus cantonensis infection by immunizing rats with somatic (L3S) and metabolic (L3ES) antigens from third-stage larvae and with somatic antigen from adult female worms (FACE) via either a combined intraperitoneal and oral (IP-OR-OR) or subcutaneous (SC-SC-SC) route. The most effective mean in inducing protection was the administration of L3S by the IP-OR-OR route, as judged from reduced mortality, prolonged survival periods and/or reduced worm burdens. Such a protocol reduced mortality to below 20% of the unimmunized controls. The worm burden of the L3S-immunized rats was only about 25% of the control value and the survival period was at least double. However, judging from gross morphology, the absence of stunted growth and unimpaired ficundity of the worms that developed into adult stage, it appears that those worms that survived had normal development. The latter observation is in contrast to that noted with FACE immunization which seemed to affect also the growth and development of worms that survived into adult stage.
通过腹腔内和口服联合（IP-OR-OR）或皮下（SC-SC-SC）途径，用来自第三期幼虫的体细胞（L3S）和代谢（L3ES）抗原以及来自成年雌虫的体细胞抗原（FACE）免疫大鼠，试图诱导对广州管圆线虫感染的获得性免疫。从降低死亡率、延长存活期和/或减少虫负荷判断，诱导保护的最有效方法是通过IP-OR-OR途径给予L3S。这样的方案将死亡率降低到未免疫对照组的20%以下。L3S免疫大鼠的虫负荷仅约为对照值的25%，存活期至少延长一倍。然而，从大体形态来看，发育为成虫阶段的虫体没有生长发育迟缓且生殖力未受损，似乎存活下来的那些虫体发育正常。后一观察结果与FACE免疫的情况形成对比，FACE免疫似乎也影响存活到成虫阶段的虫体的生长和发育。
