Antitumor Immunity Mediated by Engineered Stem Cells Exploiting TRAIL-Induced Cell Death and FLT3L Immunomodulation.

PURPOSE

Death receptor (DR)-targeted therapies offer a promising tumor cell-specific therapeutic strategy for highly malignant brain tumors, such as glioblastoma (GBM). However, whether DR-mediated cell death leads to activation of the adaptive immune system and impacts the tumor immune microenvironment (TIME) remains unknown. In this study, we explored the (i) immunomodulatory role of secretable human DR4/5 ligand, TNF-related apoptosis-inducing ligand (S-TRAIL) and (ii) the therapeutic potential of mesenchymal stem cell (SC)-delivered S-TRAIL and myeloid progenitor cell-activating cytokine, FMS-like tyrosine kinase 3 ligand (FLT3L).

EXPERIMENTAL DESIGN

We created syngeneic murine immune-active and -suppressive mouse GBM tumor models expressing a human-murine chimeric DR5. Next, we created therapeutic SCs that release FLT3L and S-TRAIL and assessed their efficacy in GBM tumor models. To facilitate clinical translation, we tested the mechanism-based efficacy of encapsulated SC-TRAIL/FLT3L in both syngeneic and humanized mouse tumor models of GBM resection.

RESULTS

We show that S-TRAIL-induced apoptosis in GBM cells provokes infiltration and maturation of dendritic cells within the TIME in vivo. Next, we show that locoregional transplantation of encapsulated bimodal SCs expressing S-TRAIL and FLT3L post surgical GBM resection improves the survival probability and induces upregulation of conventional dendritic cell type 1 and CD8+ T cells. Furthermore, treatment with encapsulated off-the-shelf clinical-grade bimodal human SCs in GBM-bearing humanized mice results in a significant decrease in tumor volumes.

CONCLUSIONS

This study uncovers the immunologic role of TRAIL-mediated cell death in the TIME and provides evidence for the encapsulated cell-based therapy to kill residual tumor cells and induce long-term immunity.