Fu Yu, Hao Xin, Nie Jingru, Zhang Hongliang, Shang Peng, Zhang Bo, Zhang Hao
State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China; Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences, Hainan, China; Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Hainan, China.
State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, China.
J Lipid Res. 2025 May;66(5):100804. doi: 10.1016/j.jlr.2025.100804. Epub 2025 Apr 15.
Lipid deposition is related to agricultural animal production and human health, and elucidating its molecular regulatory mechanisms is a topic of interest and a challenge in current scientific research. Musculoskeletal embryonic nuclear protein 1 (MUSTN1) regulates growth and development, including muscle tissue; however, its role in fat deposition remains unknown. Thus, our objective was to determine this role. Our new findings were as follows: MUSTN1 was highly expressed in the fat tissue of pigs with strong adipose deposition capacity; functionally, MUSTN1 promoted the proliferation and adipogenic differentiation of porcine and mouse preadipocytes. MUSTN1 knockout mice were protected against HFD-induced obesity, hepatic steatosis, and insulin resistance; and fatty acid binding protein 3 was identified as an interacting protein of MUSTN1, which facilitated preadipocyte proliferation and differentiation by activating the phosphatidylinositol 3 kinase/AKT signaling pathways. This study reveals a positive regulator for fat development, which suggests a novel approach for studying obesity and animal genetic improvement through the modulation of MUSTN1 expression.
脂质沉积与农业动物生产和人类健康相关，阐明其分子调控机制是当前科学研究中一个备受关注且具有挑战性的课题。肌肉骨骼胚胎核蛋白1（MUSTN1）调节生长发育，包括肌肉组织；然而，其在脂肪沉积中的作用尚不清楚。因此，我们的目标是确定这一作用。我们的新发现如下：MUSTN1在具有强大脂肪沉积能力的猪的脂肪组织中高表达；在功能上，MUSTN1促进猪和小鼠前体脂肪细胞的增殖和成脂分化。MUSTN1基因敲除小鼠对高脂饮食诱导的肥胖、肝脂肪变性和胰岛素抵抗具有保护作用；脂肪酸结合蛋白3被鉴定为MUSTN1的相互作用蛋白，其通过激活磷脂酰肌醇3激酶/AKT信号通路促进前体脂肪细胞的增殖和分化。本研究揭示了一种脂肪发育的正向调节因子，这为通过调节MUSTN1表达来研究肥胖和动物遗传改良提供了一种新方法。
