Zhou Shuaishuai, Wang Miaomiao, Chen Ruoyi, Yu Wengeng, Li Mengmeng, Meng Siwen, Zhang Ziru, Xia Congcong, Zhao Hongtao, Liu Lei
Jiangsu Key Laboratory for Eco-Agriculture Biotechnology Around Hongze Lake, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environment Protection, Huaiyin Normal University, Huai'an, 223300, China.
College of Life Science, Hebei Normal University, Hebei, 050024, China.
Plant Mol Biol. 2025 Apr 16;115(3):58. doi: 10.1007/s11103-025-01587-5.
Timely seed germination is a crucial process for plant survival and subsequent propagation, which is significantly impacted by high temperatures. ROOT INITIATION DEFECTIVE 1 (RID1), an Arabidopsis DEAH/RHA RNA helicase, has been previously reported to modulate the cellular specification of mature female gametophyte and callus initiation from hypocotyl explants through proper alternative splicing. However, the role of RID1 in the regulation of seed germination remains largely unexplored. Here, we identified that mutations in RID1 delayed seed germination more severely at 28℃ compared to 22℃. Notably, we found that the rid1-1 mutation did not significantly alter genome-wide alternative splicing patterns during seed germination compared to the wild type. Further evidences demonstrated that RID1 regulates seed germination via the abscisic acid (ABA) pathway by physically and genetically interacting with the SKIP-associated transcriptional complex. These results suggest that RID1 regulates seed germination in response to ambient temperature at the transcriptional level rather than through alternative splicing regulation. This study provides novel insights into the mechanisms underlying the regulation of seed germination.
及时的种子萌发是植物存活及后续繁殖的关键过程，这一过程会受到高温的显著影响。拟南芥DEAH/RHA RNA解旋酶ROOT INITIATION DEFECTIVE 1（RID1）此前已被报道可通过适当的可变剪接来调节成熟雌配子体的细胞特化以及下胚轴外植体的愈伤组织起始。然而，RID1在种子萌发调控中的作用在很大程度上仍未得到探索。在此，我们发现与22℃相比，RID1突变在28℃时更严重地延迟了种子萌发。值得注意的是，我们发现与野生型相比，rid1-1突变在种子萌发过程中并未显著改变全基因组的可变剪接模式。进一步的证据表明，RID1通过与SKIP相关转录复合体进行物理和遗传相互作用，经由脱落酸（ABA）途径调控种子萌发。这些结果表明，RID1在转录水平而非通过可变剪接调控来响应环境温度从而调节种子萌发。本研究为种子萌发调控机制提供了新的见解。
