Hirota Ryosuke, Sasaki Masanori, Teramoto Atsushi, Yamashita Toshihiko, Kocsis Jeffery D, Honmou Osamu
Department of Neural Regenerative Medicine, Institute of Regenerative Medicine, Sapporo Medical University School of Medicine, S1W17, Chuo-ku, Sapporo, 060-8556, Hokkaido, Japan.
Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, Sapporo, 060-8556, Japan.
Mol Brain. 2025 Apr 16;18(1):35. doi: 10.1186/s13041-025-01210-0.
Limited spontaneous recovery occurs after spinal cord injury (SCI). However, current knowledge indicates that multiple forms of axon growth in spared axons can lead to circuit reorganization. Intravenous infusion of mesenchymal stem cells (MSCs) provides functional improvements after SCI with an increased axonal network. In this study, we examined how intravenous infusion of MSCs facilitates axonal connections in the rubrospinal tract (RST), one of the significant descending tracts, using AAV neuronal tracing techniques. Our finding demonstrated that infused MSCs significantly enhanced axonal signal intensity in the RST, not only around the injury site but also in the rostral and caudal regions, suggesting that neural circuit reorganization is facilitated.
脊髓损伤（SCI）后自发恢复有限。然而，目前的知识表明， spared轴突中的多种轴突生长形式可导致神经回路重组。静脉输注间充质干细胞（MSCs）可在SCI后改善功能，并增加轴突网络。在本研究中，我们使用腺相关病毒（AAV）神经元追踪技术，研究了静脉输注MSCs如何促进红核脊髓束（RST）（重要的下行束之一）中的轴突连接。我们的研究结果表明，注入的MSCs显著增强了RST中的轴突信号强度，不仅在损伤部位周围，而且在头端和尾端区域，这表明神经回路重组得到了促进。
