一种用于检测麻痹性贝类毒素的荧光测定法和福林-酚试剂测定法联用方法。
A combination fluorescence assay and Folin-Ciocalteau phenol reagent assay for the detection of paralytic shellfish poisons.
作者信息
Mosley S, Ikawa M, Sasner J J
出版信息
Toxicon. 1985;23(3):375-81. doi: 10.1016/0041-0101(85)90021-2.
Paralytic shellfish poison (PSP) profiles of crude shellfish extracts were determined by linear gradient elution from a Bio-Rad AG-50-X4 strong cation-exchange resin mini-column. STX, GTX2 and GTX3 were detected by fluorescence assay. NeoSTX and GTX1/GTX4 were detected with a Folin-Ciocalteau phenol reagent assay. The major toxicity associated with extracts of Mytilus edulis and Mya arenaria collected during a 1972 red tide off Hampton, New Hampshire, was due to the presence of GTX1/GTX4, with some activity associated with neoSTX, GTX2 and GTX3. STX was also present. Correlations to mouse toxicity are provided.
通过在Bio-Rad AG-50-X4强阳离子交换树脂微型柱上进行线性梯度洗脱，测定了贝类粗提物中的麻痹性贝类毒素（PSP）谱。通过荧光分析法检测石房蛤毒素（STX）、膝沟藻毒素2（GTX2）和膝沟藻毒素3（GTX3）。用福林-酚试剂法检测新石房蛤毒素（NeoSTX）和膝沟藻毒素1/膝沟藻毒素4（GTX1/GTX4）。1972年新罕布什尔州汉普顿附近发生赤潮期间采集的紫贻贝和砂海螂提取物的主要毒性归因于GTX1/GTX4的存在，新石房蛤毒素、GTX2和GTX3也有一定活性。石房蛤毒素也存在。还提供了与小鼠毒性的相关性。
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