Morgan D L, Wenzel D G
Toxicol Lett. 1985 Jul;26(1):37-42. doi: 10.1016/0378-4274(85)90182-1.
The objective of this study was to determine if exposure of cultured rat lung fibroblasts to O3 would trigger a subsequent attack by rat alveolar macrophages (AM). Fibroblasts labeled with [3H]proline (3HPR) were exposed to O3 (0, 0.74, 1.35, 2.46 or 4.48 ppm) for 1 h. AM were added (5:1, AM: fibroblast) and after 2, 24, 48, 76 and 96 h of co-culture, loss of 3HPR was measured as an index of AM-induced injury. Fibroblasts exposed to O3 and co-cultured with AM lost significantly more 3HPR than without AM co-culture. The results suggest that O3 injury to the lung may be mediated in part through an attack by AM upon O3-injured fibroblasts.
本研究的目的是确定培养的大鼠肺成纤维细胞暴露于臭氧（O3）是否会引发大鼠肺泡巨噬细胞（AM）随后的攻击。用[3H]脯氨酸（3HPR）标记的成纤维细胞暴露于O3（0、0.74、1.35、2.46或4.48 ppm）1小时。加入AM（5:1，AM：成纤维细胞），共培养2、24、48、76和96小时后，测量3HPR的损失作为AM诱导损伤的指标。暴露于O3并与AM共培养的成纤维细胞比未与AM共培养的成纤维细胞损失的3HPR明显更多。结果表明，O3对肺的损伤可能部分通过AM对O3损伤的成纤维细胞的攻击来介导。
