将未发酵或发酵的脱油米糠粕添加到兔子的日粮中会影响其生长性能、养分消化率、盲肠微生物群组成和肠道屏障功能。

Incorporation of unfermented or fermented de-oiled rice bran meal into a rabbit's diet impacts growth performance, nutrient digestibility, cecal microbiota composition, and intestinal barrier function.

作者信息

Zeid Shehata, Daniel Sindaye, Jinghong Liao, Hang Suqin

机构信息

National Center for International Research on Animal Gut Nutrition, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing, China.

Department of Animal and Poultry Production, Faculty of Agriculture, Damanhur University, Damanhur, Egypt.

出版信息

Anim Biosci. 2025 Jul;38(7):1459-1474. doi: 10.5713/ab.24.0890. Epub 2025 Apr 11.

DOI:10.5713/ab.24.0890

PMID:40241585

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12229920/

Abstract

OBJECTIVE

This study assessed the effects of incorporating unfermented or fermented rice bran meal (RBM) into a rabbit's diet on their growth performance, cecal microbiota, and intestinal health.

METHODS

Twenty-one male weaned New Zealand White rabbits aged 6 weeks were randomly allocated into 3 groups (n = 7). Rabbits consumed the basal diet in the control (CON) group and the basal diet with a 20% substitution of either unfermented RBM (UFRBM) or fermented RBM (FRBM) for 8 weeks. A combination of Lactobacillus johnsonii L63 with hydrolytic enzymes ([cellulase (100 U/g], phytase [1.25 U/g], and papain [300 U/g]) was used to FRBM for 60 h at 37°C and a pH value of 4.8.

RESULTS

Our results demonstrated that the rabbits' growth performance, serum biochemical variables, and cecal microbiota for α and β diversities at the phylum level didn't differ among the treatments. The nutrient digestibility, cecal and jejunal morphology, or α-amylase and trypsin activities increased in the FRBM group than the CON and UFRBM groups, while the catalase and malondialdehyde activities decreased in the UFRBM group (p<0.05). The solute carrier families 15 and 5 levels were higher in the FRBM group than the UFRBM and CON groups, while the UFRBM group improved the levels of the solute carrier family 1, occludin, and V9D2013 group at the genus level than the FRBM and CON groups (p<0.05). The abundance of glycolysis-to-gluconeogenesis was increased in the FRBM and UFRBM groups compared to the CON group (p<0.05). The total short-chain fatty acid, acetate, and butyrate concentrations were, respectively, improved in the FRBM group than the CON and UFRBM groups (p<0.05).

CONCLUSION

The two formulas incorporating 20% of UFRBM or FRBM in rabbit diet should be recommended to farmers, particularly the FRBM, to decrease the reliance on corn and soybean meal for rabbit production.

摘要

目的

本研究评估了在兔日粮中添加未发酵或发酵米糠粕（RBM）对其生长性能、盲肠微生物群和肠道健康的影响。

方法

将21只6周龄的雄性断奶新西兰白兔随机分为3组（n = 7）。对照组（CON）的兔子食用基础日粮，另外两组分别用20%的未发酵RBM（UFRBM）或发酵RBM（FRBM）替代基础日粮中的部分成分，持续8周。将约氏乳杆菌L63与水解酶（[纤维素酶（100 U/g）、植酸酶（1.25 U/g）和木瓜蛋白酶（300 U/g）]）组合用于在37°C和pH值4.8的条件下对FRBM进行60小时的发酵。

结果

我们的结果表明，各处理组之间兔子的生长性能、血清生化指标以及盲肠微生物群在门水平上的α和β多样性没有差异。FRBM组的营养物质消化率、盲肠和空肠形态，或α-淀粉酶和胰蛋白酶活性均高于CON组和UFRBM组，而UFRBM组的过氧化氢酶和丙二醛活性降低（p<0.05）。FRBM组的溶质载体家族15和5水平高于UFRBM组和CON组，而UFRBM组在属水平上提高了溶质载体家族1、闭合蛋白和V9D2013组的水平，高于FRBM组和CON组（p<0.05）。与CON组相比，FRBM组和UFRBM组糖酵解-糖异生的丰度增加（p<0.05）。FRBM组的总短链脂肪酸、乙酸和丁酸浓度分别高于CON组和UFRBM组（p<0.05）。