Spatial metabolomics, as a frontier technology, is capable of conducting the comprehensive characterization of metabolites within organisms in terms of qualitative, quantitative and positional dimensions, so as to facilitate the visual analysis of biological processes. This paper summarizes the birth and development of spatial metabolomics, explains its differences and advantages from traditional metabolomics and summarizes its application in plant research. In addition, the limitations of spatial metabolomics are summarized and discussed, along with the technological improvement and application innovation of spatial metabolomics, in order to provide reference for the development strategy of spatial metabolomics and its application in plant research.