Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany.
Center of Plant System Biology and Biotechnology, Plovdiv, Bulgaria.
Methods Mol Biol. 2021;2264:33-46. doi: 10.1007/978-1-0716-1201-9_3.
Recent methodological advances in both gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS) have provided a deep understanding of metabolic regulation occurring in plant cells. The application of these techniques to agricultural systems is, however, subject to more complex interactions. Here we summarize a step-by-step modern metabolomics methodology that generates metabolome data toward the implementation of metabolomics in crop breeding. We describe a metabolic workflow, and provide guidelines for handling large sample numbers for the specific purpose of metabolic quantitative trait loci approaches.
近年来，气相色谱-质谱联用（GC-MS）和液相色谱-质谱联用（LC-MS）技术在代谢组学研究中取得了长足的发展，为解析植物细胞代谢调控网络提供了有力的工具。然而，将这些技术应用于农业系统还面临着许多复杂的问题。本文概述了一套现代代谢组学研究方法，旨在为作物代谢组学研究和品种改良提供参考。文中详细描述了代谢组学数据获取的基本流程，为高通量样本的代谢组学分析提供了实验方案和数据处理方法。
