Scarberry Luke, Thesing Garrett, Brennan Kevin, Williams Madison, Summers Matthew K
Department of Radiation Oncology, The Ohio State University Comprehensive Cancer Center, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.
Biomedical Sciences Graduate Program, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.
Int J Mol Sci. 2025 Mar 27;26(7):3089. doi: 10.3390/ijms26073089.
The role of p31 in deactivating the spindle assembly checkpoint is well described in the literature; however, the data are all completed using Variant 2 of p31. p31 is known to be expressed as two different splice variants: Variant 1 and Variant 2. Variant 1 contains an additional 32 N-terminal residues compared to Variant 2. We report that Variant 1 exhibits a reduced ability to bind to MAD2 and thus a reduced ability to induce mitotic progression. Additionally, we show that Variant 1 exhibits reduced stability compared to Variant 2. We further show that Variant 1 is uniquely expressed in the Testes, indicating a potentially unique role of Variant 1 in that organ. Overall, we demonstrate the N-terminus of p31 is capable of modulating p31 activity in mitosis.
p31在失活纺锤体组装检查点中的作用在文献中已有充分描述；然而，所有数据都是使用p31的变体2完成的。已知p31以两种不同的剪接变体形式表达：变体1和变体2。与变体2相比，变体1在N端额外含有32个残基。我们报告称，变体1与MAD2结合的能力降低，因此诱导有丝分裂进程的能力也降低。此外，我们表明，与变体2相比，变体1的稳定性降低。我们进一步表明，变体1仅在睾丸中表达，表明变体1在该器官中可能具有独特作用。总体而言，我们证明了p31的N端能够调节其在有丝分裂中的活性。
