McKnight A T, Hughes J, Kosterlitz H W
Proc R Soc Lond B Biol Sci. 1979 Aug 1;205(1159):199-207. doi: 10.1098/rspb.1979.0060.
A method for obtaining incorporation of labelled amino acids into enkephalins of guinea-pig striatum in vitro and isolating the labelled enkephalins is described. The incorporation of [3H]tyrosine increases with time after a delay of 2 h. Cycloheximide added during this time, but not at later stages, inhibits incorporation. The results indicate that the enkephalins are produced locally, probably by ribosomal synthesis. The lag period may be due to the time required for the synthesis of precursors and their conversion to the enkephalins.
本文描述了一种在体外将标记氨基酸掺入豚鼠纹状体脑啡肽并分离标记脑啡肽的方法。[3H]酪氨酸的掺入在延迟2小时后随时间增加。在此期间添加环己酰亚胺可抑制掺入，但在后期添加则无效。结果表明，脑啡肽可能是通过核糖体合成在局部产生的。延迟期可能是由于前体合成及其转化为脑啡肽所需的时间。
