通过间接免疫荧光法检测石蜡包埋组织和细胞培养物中的病毒颗粒

Detection of Viral Particles in Paraffinized Tissues and Cell Culture by Indirect Immunofluorescence.

作者信息

Souza Yuli, de Oliveira Barbara, Pelajo-Machado Marcelo, Manso Pedro Paulo

机构信息

Laboratory of Experimental Medicine and Health (LAMES), Oswaldo Cruz Institute (IOC), Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, RJ, Brazil.

出版信息

Methods Mol Biol. 2025;2913:141-151. doi: 10.1007/978-1-0716-4458-4_12.

DOI:10.1007/978-1-0716-4458-4_12

PMID:40249431

Abstract

Immunofluorescence enables detection of proteins in tissue and cell culture. The specific reaction between the complex antigen-antibody confers security to detection. Disclosure of proteins of interest by fluorochrome allows a perception of its spatial distribution and location. Here we describe the technique of indirect immunofluorescence for detection of E protein from yellow fever virus in paraffinized tissue and in cell cultures.

摘要

免疫荧光技术能够检测组织和细胞培养物中的蛋白质。复杂抗原-抗体之间的特异性反应为检测提供了可靠性。通过荧光染料揭示感兴趣的蛋白质，可了解其空间分布和位置。在此，我们描述了用于检测石蜡包埋组织和细胞培养物中黄热病毒E蛋白的间接免疫荧光技术。