Leibel R L, Hirsch J, Berry E M, Gruen R K
Am J Clin Nutr. 1985 Aug;42(2):198-206. doi: 10.1093/ajcn/42.2.198.
Using a newly developed in vitro technique, the rate of re-esterification of lipolyzed free fatty acids (FFA) in small fragments of human subcutaneous adipose tissue was measured. When related to simultaneous glycerol release, this measure permits the calculation of the molar ratios of glycerol and FFA leaving the adipocyte. In weight-stable, never-obese control subjects the molar ratio of FFA:glycerol leaving the adipocytes is 1.4:1. During fasting, this ratio climbs to 2.7:1, close to the theoretical maximum of 3.0:1. Adipocytes from weight-stable obese subjects do not differ significantly from adipocytes of control in regard to this ratio. However, the adipocytes of weight-stable reduced-obese (RO) subjects display a significantly higher FFA:glycerol ratio than the adipocytes of either control or obese subjects. The presence of this fasting-like physiology in adipose tissue from weight-stable RO subjects is of particular interest since these same individuals have other systemic metabolic and subjective findings compatible with caloric deprivation.
采用一种新开发的体外技术，测定了人皮下脂肪组织小片段中脂解游离脂肪酸（FFA）的再酯化率。与同时释放的甘油相关时，该测量值可用于计算离开脂肪细胞的甘油和FFA的摩尔比。在体重稳定、从未肥胖的对照受试者中，离开脂肪细胞的FFA:甘油摩尔比为1.4:1。禁食期间，该比例升至2.7:1，接近理论最大值3.0:1。体重稳定的肥胖受试者的脂肪细胞在该比例方面与对照受试者的脂肪细胞无显著差异。然而，体重稳定的减重肥胖（RO）受试者的脂肪细胞显示出比对照或肥胖受试者的脂肪细胞显著更高的FFA:甘油比例。体重稳定的RO受试者脂肪组织中这种类似禁食的生理状态的存在尤其令人感兴趣，因为这些个体还有其他与热量不足相符的全身代谢和主观表现。
