Kontro Hilkka, McGlory Chris, MacInnis Martin J
Faculty of Kinesiology, Human Performance Lab, University of Calgary, Calgary, Alberta, Canada.
School of Kinesiology and Health Studies, Queen's University, Kingston, Ontario, Canada.
Physiol Rep. 2025 Apr;13(8):e70311. doi: 10.14814/phy2.70311.
We employed deuterated water (HO) to compare the fractional synthetic rate (FSR) of hemoglobin in trained (n = 10) and untrained humans (n = 10). Participants had a mean V̇Omax of 49.8 [SD: 10.9] mL/kg/min, hemoglobin mass of 775 [180] g, and red blood cell volume of 2370 [550] mL. After a loading dose, participants ingested HO daily for 28 days to maintain a stable H body water enrichment (~0.5 atom percent excess (APE)), as measured in saliva samples. H-enriched alanine APE was measured in RBC protein using gas chromatography pyrolysis isotope ratio mass spectrometry. The increase in APE for Hb protein was nonlinear for the first 2 weeks but stabilized from day 14 to day 28, with mean APE reaching 0.033 [0.005]% on day 28. Hb FSR calculated over this 2-week period was 0.84 [0.15]%/day, which equated to an Hb absolute synthetic rate of 6.5 [2.2] g/day and a lifespan of 126 [30] days. Hb FSR was not different between trained (0.83 [0.19] %/day) and untrained (0.86 [0.24] %/day, p = 0.81) individuals or between males (0.80 [0.25] %/day) and females (0.88 [0.17] %/day, p = 0.24). Endurance training and sex did not affect Hb FSR. The HO method to measure Hb FSR is feasible in humans.
我们使用重水（H₂O）比较训练有素的人（n = 10）和未受过训练的人（n = 10）血红蛋白的合成率（FSR）。参与者的平均最大摄氧量为49.8 [标准差：10.9] mL/kg/分钟，血红蛋白质量为775 [180] g，红细胞体积为2370 [550] mL。在给予负荷剂量后，参与者每天摄入重水，持续28天，以维持稳定的体内水富集（约0.5原子百分比过剩（APE）），这是通过唾液样本测量的。使用气相色谱热解同位素比质谱法测量红细胞蛋白质中富集氢的丙氨酸的APE。血红蛋白蛋白质的APE在前2周呈非线性增加，但从第14天到第28天趋于稳定，第28天的平均APE达到0.033 [0.005]%。在此2周期间计算的血红蛋白FSR为0.84 [0.15]%/天，这相当于血红蛋白绝对合成率为6.5 [2.2] g/天，寿命为126 [30]天。训练有素的人（0.83 [0.19] %/天）和未受过训练的人（0.86 [0.24] %/天，p = 0.81）之间或男性（0.80 [0.25] %/天）和女性（0.88 [0.17] %/天，p = 0.24）之间的血红蛋白FSR没有差异。耐力训练和性别不影响血红蛋白FSR。用重水法测量血红蛋白FSR在人体中是可行的。
