Delaying quality deterioration with multifunctional gelatin-based film by inhibiting microbial growth in fresh-cut navel oranges.

Maintaining the quality of fresh-cut navel oranges during storage remains a significant challenge due to microbial infestation. This study aimed to investigate the efficacy of a multifunctional gelatin-based (MG) film in retaining flavor, delaying quality deterioration, and extending the shelf life of fresh-cut navel oranges. Basic quality indicators demonstrated that MG film packaging effectively maintained the appearance and texture of fresh-cut navel oranges, reduced water loss and microbial growth, and extended the freshness period compared to unpacked samples and PE film. Additionally, the MG film packaging delayed a decline in the β-glucosidase (β-GC) activity, reduced fruit softening and flavor degradation, and maintained the volatile organic compounds (VOCs) content, particularly limonene and myrcene. The non-targeted metabolomics results showed that MG film significantly inhibited the degradation of organic acids, flavonoids, and sugars, further slowing the deterioration process. Furthermore, the MG film inhibited spoilage fungi (Penicillium, Aspergillus, and Pseudomonas) and foodborne bacteria such as Pantoea, Enterobacteriaceae, and Gluconobacter by restricting microbial nutrient utilization and metabolic activity, which modulated the microbial community structure and greatly delayed spoilage processes. This study highlighted the potential of MG film in delaying quality deterioration and extending the shelf life of fresh-cut fruits by inhibiting microbial growth.