Tootle Tina L
Biology, University of Iowa, Iowa City, Iowa, United States.
Anatomy and Cell Biology, University of Iowa Carver College of Medicine, Iowa City, Iowa, United States.
MicroPubl Biol. 2025 Apr 3;2025. doi: 10.17912/micropub.biology.001571. eCollection 2025.
Expression of GFP-Actin results in nuclear actin rod formation during specific stages of oogenesis. Loss of prostaglandin (PG) synthesis and signaling results in an increased frequency of cells with nuclear actin rods; there are less rods per cell, but the rods are longer. These findings suggest that loss of PGs results in increased nuclear actin and are consistent with prior findings assessing the roles of PGs in modulating endogenous nuclear actin. Thus, GFP-Actin rod formation can be used as a tool to screen for new regulators of nuclear actin.
绿色荧光蛋白-肌动蛋白（GFP-Actin）的表达在卵子发生的特定阶段会导致核肌动蛋白杆的形成。前列腺素（PG）合成及信号传导的缺失会导致带有核肌动蛋白杆的细胞频率增加；每个细胞中的杆较少，但杆更长。这些发现表明PGs的缺失会导致核肌动蛋白增加，并且与之前评估PGs在调节内源性核肌动蛋白中作用的研究结果一致。因此，GFP-肌动蛋白杆的形成可作为筛选核肌动蛋白新调节因子的工具。
