Chemnitius J M, Burger W, Bing R J
Am J Physiol. 1985 Aug;249(2 Pt 2):H285-92. doi: 10.1152/ajpheart.1985.249.2.H285.
Krebs-Henseleit buffer (KH) and a perfluorochemical (FC-43) were compared as perfusates in an isolated working rabbit heart preparation. Both perfusates were oxygenated in an identical manner using an infant bubble oxygenator. After 60 min of perfusion, no difference could be detected in the ratio of wet to dry heart weight between KH- and FC-43-perfused hearts (KH, 6.25 +/- 0.3; FC-43, 5.99 +/- 0.20). Left ventricular systolic pressure, maximal rate of left ventricular pressure rise, mean aortic systolic pressure, cardiac output, aortic flow, left ventricular power, and myocardial O2 consumption (MVO2) were significantly higher in FC-43-perfused hearts throughout the time of perfusion. However, there were no differences in resistance to cardiac output and heart rate. In KH- and FC-43-perfused hearts, MVO2 and left ventricular power were closely correlated (KH, r = 0.793; FC-43, r = 0.831). Significantly higher coronary flow of KH-perfused hearts could be attributed to the lower viscosity of KH (1.05 Pa . s) compared with FC-43 (1.91 Pa . s). Increased O2 extraction during KH perfusion could not compensate for low O2-carrying capacity of KH buffer (345 compared with 705 nmol O2 X ml-1 in FC-43 emulsion). A postischemic increase of coronary flow was observed only in FC-43-perfused hearts (28%). These results demonstrate a different response of perfused heart preparations to FC-43 and KH buffer.
在离体工作兔心制备中，比较了 Krebs - Henseleit 缓冲液（KH）和全氟化合物（FC - 43）作为灌注液的效果。两种灌注液均使用婴儿气泡氧合器以相同方式进行氧合。灌注 60 分钟后，KH 灌注组和 FC - 43 灌注组心脏的湿重与干重之比无差异（KH 组为 6.25±0.3；FC - 43 组为 5.99±0.20）。在整个灌注过程中，FC - 43 灌注组心脏的左心室收缩压、左心室压力上升最大速率、平均主动脉收缩压、心输出量、主动脉流量、左心室功率和心肌耗氧量（MVO₂）均显著更高。然而，心输出量阻力和心率并无差异。在 KH 灌注组和 FC - 43 灌注组心脏中，MVO₂与左心室功率密切相关（KH 组，r = 0.793；FC - 43 组，r = 0.831）。KH 灌注组心脏冠状动脉血流量显著更高，这可归因于 KH 的粘度（1.05 Pa·s）低于 FC - 43（1.91 Pa·s）。KH 灌注期间氧提取增加无法弥补 KH 缓冲液的低携氧能力（FC - 43 乳剂中为 705 nmol O₂·ml⁻¹，而 KH 缓冲液中为 345 nmol O₂·ml⁻¹）。仅在 FC - 43 灌注组心脏中观察到缺血后冠状动脉血流量增加（28%）。这些结果表明灌注心脏制剂对 FC - 43 和 KH 缓冲液有不同反应。
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