Rahamathulla P M, Watanabe K, Ashraf M, Millard R W
Exp Pathol. 1985;28(3):157-65. doi: 10.1016/s0232-1513(85)80003-7.
The ability of an oxygenated perfluorocarbon (PFC) emulsion to prevent early signs of myocardial cell stress associated with Krebs-Henseleit (KH) perfusion was evaluated in isolated rat hearts supported in a Langendorff apparatus. Throughout the two-hour perfusion, hearts in both perfusion groups had similar left ventricular pressure and rates of left ventricular pressure development (dP/dt). After 2 h, coronary perfusate flow was 6.6 +/- 1.0 ml/min in the KH group and 2.2 +/- 0.1 ml/min in the PFC group. Oxygen content was 1.5 +/- 0.1 ml/100 ml and 3.4 +/- 0.1 ml/100 ml, in KH and PFC groups, respectively. Perfusate lactate levels rose from zero to 24.0 +/- 8.0 microgram/ml in the KH group and to 10.0 +/- 3.0 micrograms/ml in the PFC group. Reduction in myocardial cell injury and in total calcium content was also observed in hearts perfused with PFC emulsion. At the electron microscopic level, mitochondrial structure was preserved in both normal and injured myocytes of hearts perfused with PFC. We conclude that early signs of anaerobic metabolism are retarded by perfusion with PFC emulsions and that ventricular contractile performance is maintained at approximately one-half coronary perfusate flow in the PFC hearts. The mechanism whereby coronary flow is regulated downward in the PFC perfused hearts remains unanswered.
在Langendorff装置支持的离体大鼠心脏中，评估了含氧全氟碳（PFC）乳剂预防与Krebs-Henseleit（KH）灌注相关的心肌细胞应激早期迹象的能力。在整个两小时的灌注过程中，两个灌注组的心脏左心室压力和左心室压力发展速率（dP/dt）相似。两小时后，KH组的冠状动脉灌注液流量为6.6±1.0 ml/min，PFC组为2.2±0.1 ml/min。KH组和PFC组的氧含量分别为1.5±0.1 ml/100 ml和3.4±0.1 ml/100 ml。KH组灌注液乳酸水平从零升至24.0±8.0微克/毫升，PFC组升至10.0±3.0微克/毫升。在用PFC乳剂灌注的心脏中，还观察到心肌细胞损伤和总钙含量的降低。在电子显微镜水平上，用PFC灌注的心脏的正常和受损心肌细胞中的线粒体结构均得以保留。我们得出结论，PFC乳剂灌注可延缓无氧代谢的早期迹象，并且在PFC灌注的心脏中，心室收缩性能在冠状动脉灌注液流量约为一半时得以维持。PFC灌注的心脏中冠状动脉血流向下调节的机制仍未得到解答。
