Ansarin Atefeh, Shanehbandi Dariush, Zarredar Habib, Ostadrahimi Alireza, Gilani Neda, Ansarin Khalil
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Bioimpacts. 2024 Oct 2;15:30593. doi: 10.34172/bi.30593. eCollection 2025.
Given the well-established association between metabolic syndrome (MetS) and obesity, this study elucidates the influences of sleep duration and weight on MetS risk and explores the potential role of miRNAs as underlying mechanisms.
According to sleep logs and biochemistry tests, this study investigated the association between MetS and its components, sleep duration, and weight in four subgroups: A: normal sleepers with normal weight (N = 145), B: normal sleepers with obesity (N = 140), C: short sleepers with normal weight (N = 130), and D: short sleepers with obesity (N = 142). Chi-square, one-way ANOVA, and Tukey's post hoc tests were used for statistical analysis. Furthermore, following total RNA isolation by TRIzol from blood samples, cDNA was synthesized using stem-loop technique. Quantitative real-time polymerase chain reaction (qRT-PCR) was then employed to evaluate the expression levels of miR-33a, miR-378a, miR-132-3p, and miR-181d. The data were analyzed using one-way ANOVA.
Our findings revealed the strongest association between MetS prevalence and individuals in group D (short sleepers with obesity; Cramer's V = 0.649, < 0.001). This observation underscores the synergistic effect of short sleep and obesity on MetS risk. Furthermore, there was an independent association between short sleep duration and elevated triglyceride levels ( < 0.05). MicroRNA expression analysis revealed downregulation of miR-33a and miR-181d in B, C, and D groups compared to the normal group. Conversely, miR-132-3p expression was upregulated in the B, C, and D groups.
Short sleep and obesity synergistically elevate MetS risk, potentially via miR-33a and miR-181d downregulation and miR-132-3p upregulation, impacting triglyceride metabolism.
鉴于代谢综合征（MetS）与肥胖之间已确立的关联，本研究阐明了睡眠时间和体重对MetS风险的影响，并探讨了微小RNA（miRNA）作为潜在机制的作用。
根据睡眠记录和生化测试，本研究在四个亚组中调查了MetS与其组成成分、睡眠时间和体重之间的关联：A组：体重正常的正常睡眠者（N = 145），B组：肥胖的正常睡眠者（N = 140），C组：体重正常的短睡眠者（N = 130），以及D组：肥胖的短睡眠者（N = 142）。采用卡方检验、单因素方差分析和Tukey事后检验进行统计分析。此外，通过TRIzol从血液样本中分离总RNA后，使用茎环技术合成cDNA。然后采用定量实时聚合酶链反应（qRT-PCR）评估miR-33a、miR-378a、miR-132-3p和miR-181d的表达水平。数据采用单因素方差分析进行分析。
我们的研究结果显示，MetS患病率与D组个体（肥胖的短睡眠者）之间的关联最强（克莱姆相关系数V = 0.649，P < 0.001）。这一观察结果强调了短睡眠和肥胖对MetS风险的协同作用。此外，短睡眠时间与甘油三酯水平升高之间存在独立关联（P < 0.05）。微小RNA表达分析显示，与正常组相比，B、C和D组中miR-33a和miR-181d表达下调。相反，B、C和D组中miR-132-3p表达上调。
短睡眠和肥胖协同增加MetS风险，可能是通过下调miR-33a和miR-181d以及上调miR-132-3p，影响甘油三酯代谢。
